Comparative Gene-Expression Analysis of Alzheimer’s Disease Progression with Aging in Transgenic Mouse Model
Abstract
:1. Introduction
2. Results
2.1. Amyloid Precursor Protein Processing
2.2. Tau Pathology
2.3. Endoplasmic Reticulum (ER) Stress
2.4. Calcium Signaling Disruption
2.5. Mitochondrial Dysfunction
2.6. Inflammation and Apoptosis
2.7. AD Onset with Age Progression
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. RNA Isolation and cDNA Synthesis
4.3. Gene-Expression Analysis
4.4. Statistics and Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Gene Name | Reverse Primer Sequence 5′ to 3′ | Forward Primer Sequence 5′ to 3′ |
---|---|---|
Adam10 | atcgaatcctgccatttcac | agccagagttgtgcgttttt |
Abad | gacttccagcgggttatcaa | cagtgtcatgcccactatgc |
Aph1B | gctgttcaggctcgcatatt | aatcaccatgaatgcccact |
Apo-E | gtgctgttggtcacattgct | cagtgccgtcagttcttgtg |
App | ggccctcgagaattacatca | gttcatgcgctcgtagatca |
Atf6 | ggccagactgttttgctctc | cccatacttctggtggcact |
Serca1 | tggccgatgataacttctcc | gagcccatcagtcaccaagt |
Bace1 | tttgtggagatggtggacaa | tacacaccctttcggaggtc |
Bad | gggatggaggaggagcttag | cccaccaggactggataatg |
Bid | tcacagacctgctggtgttc | gtctggcaatgttgtggatg |
Vdcc | cgttctcatcctgctcaaca | tatgctcccaatgacgatga |
Calm1 | actgggtcagaacccaacag | gttctgccgcactgatgtaa |
Caln | cagagggtgcttcgattctc | aaggcccacaaatacagcac |
Casp12 | ttcccaggaacagctgagtt | tcacgtggacaaagcttcag |
Casp3 | tgtcatctcgctctggtacg | tcccataaatgaccccttca |
Casp7 | tttgcttactccacggttcc | cacgggatctgcttcttctc |
Casp8 | ggcctccatctatgacctga | gcagaaagtctgcctcatcc |
Casp9 | aagaccatggctttgaggtg | aagtccctttcgcagaaaca |
Cdk5 | gtccatcgacatgtggtcag | acgacgttcaccaaggatgt |
P25 | cgtccactagtgagctgctg | cccacctcagaggagatgac |
Cxi | ctctccccagtaccctcgac | gggagtgggcctgaaattag |
Cxii | cctttgggaaccacagctaa | tcaaagttcccaggaagcag |
Cxiii | gttcgcagtcatagccacag | tagggccgcgataataaatg |
Cxiv | gtgtccccactgatgaggag | cagccaaaaccagatgacag |
Cxv | gaaactggaccaggtggaga | gataccctgggtgttgccta |
Cycs | gggaggcaagcataagactg | tctgccctttctcccttctt |
Perk | tggtgactgctatggaccaa | gttccatctgggtgctgaat |
Fadd | acaatgtggggagagactgg | aggtcagccaccagattcag |
Fas | ttgcaagacatgtcggaaag | cctgcatggcagttacacac |
Appbp1 | gcagccagggaagatactca | tcttctccgctgaccagatt |
Gapdh | aagggctcatgaccacagtc | acacattgggggtaggaaca |
Gq | cacgctcaagatcccataca | ggctacacggtccaagtcat |
Nmdar | cagcaggactggtcacagaa | tttgttccccaagagtttgc |
Gsk3b | gaggagagcccaatgtttca | aatttgctcccttgttggtg |
Ide | gaggcgttccaaaaacacat | gacagccaacatttcctggt |
Il1b | gaccttccaggatgaggaca | tccattgaggtggagagctt |
Ip3r | gaatttccttcgttgccaaa | cgatgcagttctggttctca |
Ire1a | cccaaatgtgatccgctact | agaatgttgtggggcttcag |
Lpl | ttttctgggactgaggatgg | gtcaggccagctgaagtagg |
Lrp1 | gacagcaaacgaggcctaag | acaggggttggtcacttcag |
Erk | tccttttgagcaccagacct | agcagatgtggtcattgctg |
Mapt | gtggccaggtggaagtaaaa | gtggagatgtgtccccagac |
Ncstn | ctgaccactctggctccttc | gctgctgaagttggttcctc |
Nep | aggcggacaacctctactca | cgaggctggtcaaaatgaat |
Nos1 | agcacctaccagctcaagga | atagtgatggccgacctgag |
Plcb1 | catccaggaggtggttcagt | ccctttcatggcttcctgta |
Psen1 | cctcatggccctggtattta | tcagccatattcaccaacca |
Pen-2 | cgggtatccaatgaggagaa | gcgagaatgatcacccagaa |
Ryr3 | gtgcagcctctactcccttg | atgtcctccaccttgtctgg |
Snca | ggagtgacaacagtggctga | caggcatgtcttccaggatt |
Tnf | cgtcagccgatttgctatct | cggactccgcaaagtctaag |
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Abid, N.B.; Naseer, M.I.; Kim, M.O. Comparative Gene-Expression Analysis of Alzheimer’s Disease Progression with Aging in Transgenic Mouse Model. Int. J. Mol. Sci. 2019, 20, 1219. https://doi.org/10.3390/ijms20051219
Abid NB, Naseer MI, Kim MO. Comparative Gene-Expression Analysis of Alzheimer’s Disease Progression with Aging in Transgenic Mouse Model. International Journal of Molecular Sciences. 2019; 20(5):1219. https://doi.org/10.3390/ijms20051219
Chicago/Turabian StyleAbid, Noman Bin, Muhammad Imran Naseer, and Myeong Ok Kim. 2019. "Comparative Gene-Expression Analysis of Alzheimer’s Disease Progression with Aging in Transgenic Mouse Model" International Journal of Molecular Sciences 20, no. 5: 1219. https://doi.org/10.3390/ijms20051219