Identification of Premeiotic, Meiotic, and Postmeiotic Cells in Testicular Biopsies Without Sperm from Sertoli Cell-Only Syndrome Patients
Abstract
:1. Introduction
2. Results
2.1. Hormone Levels in Biopsies without Sperm from SCOS Patients
2.2. Immunofluorescence Staining of Premeiotic Markers in Testicular Biopsies from Patients with Hypospermatogenesis and SCOS
2.3. Immunofluorescence Staining and RNA Expression of Premeiotic, Meiotic, and Postmeiotic Markers of Cells Isolated from Human Testicular Biopsies of Patients with Hypospermatogenesis and SCOS
2.4. Expression of Spermatogenic Markers in Isolated Cells from Testicular Biopsies of SCOS Patients after In Vitro Culture
3. Discussion
4. Materials and Methods
4.1. Human Testis Material
4.2. Testicular Cell Isolation and Culture
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Hormone (HR) Pt# | FSH (0.7–11.0 mlU/mL) | LH (0.8–8.0 mlU/mL) | ProL (1–18 ng/mL) | Test. (2.45–12.0ng/mL) |
---|---|---|---|---|
1 | 19.6 | 4.8 | 6 | 1.96 |
3 | 22.5 | 15.8 | 13 | 4.3 |
4 | 14.5 | 31.9 | 7 | 4.4 |
5 | 14.4 | 35.9 | 17 | 4.3 |
6 | 16.3 | 7.4 | 41 | 1.7 |
7 | 8.9 | 5.4 | 5 | 5.52 |
9 | 30.9 | 8.9 | 35 | 5.77 |
Biopsy# | Pre-Meiotic Markers | Meiotic Markers | Post-Meiotic | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
# | OCT4 | PLZF | VASA | GFR | CD9 | α-6-INT | SALL4 | cKIT | CREM | LDH | BOULE | PROTAMINE | ACROSIN | |||||||||||||
IF | R | IF | R | IF | R | IF | R | IF | R | IF | R | IF | R | IF | R | IF | R | IF | R | IF | R | IF | R | IF | R | |
1L | − | − | − | + | − | − | − | − | ||||||||||||||||||
1R | + | − | + | − | − | − | − | − | ||||||||||||||||||
3 | − | + | + | + | − | + | − | − | − | − | − | − | + | − | + | − | + | − | + | + | − | − | + | − | + | |
4 | + | + | − | − | + | + | + | + | − | + | + | + | − | − | + | − | − | + | − | + | − | − | ||||
5 | + | − | − | + | + | − | + | − | − | − | − | − | − | − | ||||||||||||
6 | + | + | + | − | − | + | + | + | + | + | + | − | + | − | ||||||||||||
7L | + | + | − | + | + | + | + | + | + | − | + | + | − | + | − | − | − | + | − | + | − | − | − | + | − | + |
7R | + | + | − | + | + | + | − | − | − | + | − | − | + | + | − | + | + | + | + | − | − | − | − | + | − | + |
9 | − | + | − | + | − | + | − | − | − | − | − | + | − | − | + | + | − | − | − | + | − | + | − | + | − | − |
Biopsy# | BC/AC | weeks | Pre-Meiotic Markers | Meiotic Markers | Post-Meiotic | Colonies | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
OCT4 | PLZF | VASA | GFR | CD9 | α-6-INT | SALL4 | cKIT | CREM | LDH | BOULE | PROTAMINE | ACROSIN | ||||||||||||||||||
IF | R | IF | R | IF | R | IF | R | IF | R | IF | R | IF | IF | R | IF | R | IF | R | IF | R | IF | R | IF | R | IF | |||||
4 | BC | + | +* | − | − | + | −† | + | + | − | + | +* | + | − | − | +* | − | − | + | − | +* | − | −† | |||||||
AC | 7w | − | +* | − | + | − | + | + | +† | − | − | − | + | − | +* | − | − | + | +* | − | − | − | - | − | +* | − | +† | C | ||
5 | BC | + | − | − | + | − | + | − | − | − | − | − | − | − | ||||||||||||||||
AC | 7w | − | − | − | − | − | − | − | − | − | − | − | − | − | C, S, M | |||||||||||||||
6 | BC | + | + | + | − | + | + | + | + | + | + | − | + | − | ||||||||||||||||
AC | 4w | − | − | − | − | − | − | − | − | − | − | − | − | − | S | |||||||||||||||
7L | BC | +* | + | − | + | +* | + | +* | + | + | − | +* | + | − | + | − | − | − | + | −† | + | −† | − | − | + | −† | + | |||
AC | 6w | +* | − | +* | +* | − | +* | − | − | − | +† | +† | − | +† | S, M | |||||||||||||||
7R | BC | + | + | −† | + | + | + | − | + | − | + | − | − | + | + | −† | + | +* | + | + | − | − | − | −† | + | − | + | |||
AC | 6w | − | +† | − | − | − | − | − | +† | +* | − | − | +† | − | C, S | |||||||||||||||
9 | BC | − | + | − | +* | − | +* | − | + | − | − | - | + | − | − | + | + | − | − | − | + | − | +* | − | + | − | - | |||
AC | 3w | − | +* | +* | − | − | − | − | − | − | - | +* | − | - | S, M |
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Abofoul-Azab, M.; Lunenfeld, E.; Levitas, E.; Zeadna, A.; Younis, J.S.; Bar-Ami, S.; Huleihel, M. Identification of Premeiotic, Meiotic, and Postmeiotic Cells in Testicular Biopsies Without Sperm from Sertoli Cell-Only Syndrome Patients. Int. J. Mol. Sci. 2019, 20, 470. https://doi.org/10.3390/ijms20030470
Abofoul-Azab M, Lunenfeld E, Levitas E, Zeadna A, Younis JS, Bar-Ami S, Huleihel M. Identification of Premeiotic, Meiotic, and Postmeiotic Cells in Testicular Biopsies Without Sperm from Sertoli Cell-Only Syndrome Patients. International Journal of Molecular Sciences. 2019; 20(3):470. https://doi.org/10.3390/ijms20030470
Chicago/Turabian StyleAbofoul-Azab, Maram, Eitan Lunenfeld, Eliahu Levitas, Atif Zeadna, Johnny S. Younis, Shalom Bar-Ami, and Mahmoud Huleihel. 2019. "Identification of Premeiotic, Meiotic, and Postmeiotic Cells in Testicular Biopsies Without Sperm from Sertoli Cell-Only Syndrome Patients" International Journal of Molecular Sciences 20, no. 3: 470. https://doi.org/10.3390/ijms20030470