PPP3CB Inhibits Migration of G401 Cells via Regulating Epithelial-to-Mesenchymal Transition and Promotes G401 Cells Growth
Abstract
:1. Introduction
2. Results
2.1. PPP3CB is an Important Regulator in Kidney
2.2. PPP3CB Suppresses EMT of G401 Cells
2.3. PPP3CB Inhibits Migration of G401 Cells
2.4. PPP3CB Promotes Cell Proliferation
2.5. PPP3CB 1-401 Containing the Catalytic Domain Plays a Critical Role in EMT and Cell Proliferation
3. Discussion
4. Materials and Methods
4.1. Plasmids Construction
4.2. Cell Culture and Transfection
4.3. Construction of Stable Cell Lines
4.4. RNA Extraction and Real-Time PCR
4.5. Western Blotting
4.6. Scratch Wound Healing Assay
4.7. Migration Assay
4.8. MTT Assay
4.9. 5-Ethynyl-21-Deoxyuridine (EdU) Assay
4.10. Flow Cytometry Assay
4.11. Nude Mice Tumorigenesis
4.12. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
Abbreviations
EMT | Epithelial-mesenchymal transition |
PPP | Phosphoprotein phosphatase |
PP1 | Protein Phosphatases1 |
PP2A | Protein Phosphatases2A |
PP2B | Protein Phosphatases2B |
CsA | cyclosporineA |
QPCR | Quantitative polymerase chain reaction |
NB | neuroblastoma |
FITC | fluoresceine isothiocyanate |
PI | propidium iodide |
Appendix A
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Chen, L.; He, Q.; Liu, Y.; Wu, Y.; Ni, D.; Liu, J.; Hu, Y.; Gu, Y.; Xie, Y.; Zhou, Q.; et al. PPP3CB Inhibits Migration of G401 Cells via Regulating Epithelial-to-Mesenchymal Transition and Promotes G401 Cells Growth. Int. J. Mol. Sci. 2019, 20, 275. https://doi.org/10.3390/ijms20020275
Chen L, He Q, Liu Y, Wu Y, Ni D, Liu J, Hu Y, Gu Y, Xie Y, Zhou Q, et al. PPP3CB Inhibits Migration of G401 Cells via Regulating Epithelial-to-Mesenchymal Transition and Promotes G401 Cells Growth. International Journal of Molecular Sciences. 2019; 20(2):275. https://doi.org/10.3390/ijms20020275
Chicago/Turabian StyleChen, Lei, Qingling He, Yamin Liu, Yafei Wu, Dongsheng Ni, Jianing Liu, Yanxia Hu, Yuping Gu, Yajun Xie, Qin Zhou, and et al. 2019. "PPP3CB Inhibits Migration of G401 Cells via Regulating Epithelial-to-Mesenchymal Transition and Promotes G401 Cells Growth" International Journal of Molecular Sciences 20, no. 2: 275. https://doi.org/10.3390/ijms20020275