Next Article in Journal
Suppressing Antibacterial Resistance: Chemical Binding of Monolayer Quaternary Ammonium Salts to Polymethyl Methacrylate in an Aqueous Solution and Its Clinical Efficacy
Next Article in Special Issue
Low Molecular Seleno-Aminopolysaccharides Protect the Intestinal Mucosal Barrier of Rats under Weaning Stress
Previous Article in Journal
Natural Products as Modulators of the Proteostasis Machinery: Implications in Neurodegenerative Diseases
Previous Article in Special Issue
Electrospun Chitosan/Poly (Vinyl Alcohol)/Graphene Oxide Nanofibrous Membrane with Ciprofloxacin Antibiotic Drug for Potential Wound Dressing Application
 
 
Article
Peer-Review Record

Increased ROS Scavenging and Antioxidant Efficiency of Chlorogenic Acid Compound Delivered via a Chitosan Nanoparticulate System for Efficient In Vitro Visualization and Accumulation in Human Renal Adenocarcinoma Cells

Int. J. Mol. Sci. 2019, 20(19), 4667; https://doi.org/10.3390/ijms20194667
by Revathi Kavi Rajan 1,2, Mohd Zobir Hussein 3, Sharida Fakurazi 4, Khatijah Yusoff 5 and Mas Jaffri Masarudin 1,2,3,*
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Int. J. Mol. Sci. 2019, 20(19), 4667; https://doi.org/10.3390/ijms20194667
Submission received: 11 August 2019 / Revised: 23 August 2019 / Accepted: 25 August 2019 / Published: 20 September 2019
(This article belongs to the Special Issue Chitosan Functionalizations, Formulations and Composites)

Round 1

Reviewer 1 Report

Increased ROS scavenging and antioxidant efficiency of chlorogenic acid compound delivered via a chitosan nanoparticulate system for efficient in vitro visualization and accumulation in human renal adenocarcinoma cells

In the present work " Increased ROS scavenging and antioxidant efficiency of chlorogenic acid compound delivered via a chitosan nanoparticulate system for efficient in vitro visualization and accumulation in human renal adenocarcinoma cells " in this study, a stable, monodispersed, non-toxic CNP synthesized via ionic gelation method  at  an  optimum  parameter  (600  µL  of  0.5  mg/mL  of  chitosan  and  200  µL  of  0.7  mg/mL  of  tripolyphosphate), denoted as CNP°, was used to encapsulate CGA. Sequence of physicochemical  analyses  and  morphological  studies  were  performed  to  discern  the  successful  formation  of  the CNP°-CGA  hybrid.  Antioxidant  property  in vitro antiproliferative activity of CNP°-CGA, and in vitro accumulation of fluorescently labeled  CNP°-CGA  in  cancer  cells  were  evaluated.

Findings  revealed  that  successful  formation of CNP°-CGA hybrid was reveled through an increase in particle size 134.44 ± 18.29 nm  (polydispersity index (PDI) 0.29 ± 0.03) as compared to empty CNP°, 80.89 ± 5.16 nm (PDI 0.26 ±  0.01) with a maximal of 12.04 µM CGA loaded per unit weight of CNP° using 20 µM of CGA. This  result correlated with Fourier Transform Infrared (FTIR) spectroscopic analysis, transmission (TEM)  and field emission scanning (FESEM) electron microscopy, and ImageJ evaluation.

The manuscript is well written and results are well discussed. The authors have been presented an interesting topic and describes clearly the experimental methods and results. Moreover, the paper also uses a good English. Therefore, it may recommended for publication after some modifications:

Results, section 2. Page 11, line 307 . Please add the reference “Chlorogenic acid/PEG-based organic-inorganic hybrids: A versatile sol-gel synthesis route for new bioactive materials , MATERIAL SCIENCE and ENGENEERING C 100 (2019) 837-844

Author Response

Response to Reviewer 1 Comments

Point 1: Results, section 2. Page 11, line 307 . Please add the reference “Chlorogenic acid/PEG-based organic-inorganic hybrids: A versatile sol-gel synthesis route for new bioactive materials , MATERIAL SCIENCE and ENGENEERING C 100 (2019) 837-844

Response 1: I have added the suggested citation #123

Reviewer 2 Report

The authors investigated the development of chitosan nanoparticles encapsulating chlorogenic acid for in vitro visualization and accumulation in human renal cancer cells. From the results of this study, the authors showed the proof-of-concept for the intracellular accumulation, antiproliferative activity, and antioxidant properties of chitosan nanoparticles. Thus, it will be suited for this publication with some minor concern:

In Figure 2, some of results (error bars) was cut-off from the figures. The authors must correct them. In Figure11, the authors must insert scale bar.

Author Response

Point 1: In Figure 2, some of results (error bars) was cut-off from the figures. The authors must correct them. In Figure11, the authors must insert scale bar.

Response 1: I have adjusted the error bars and included scale bar in Figure 11

Back to TopTop