Endogenous n-3 Polyunsaturated Fatty Acids Are Beneficial to Dampen CD8+ T Cell-Mediated Inflammatory Response upon the Viral Infection in Mice
Abstract
:1. Introduction
2. Results
2.1. The Expansion of Splenocytes in FAT-1 Mice Was Limited upon Acute LCMV Infection
2.2. Anti-Viral CD8+ T Cell Responses Were Suppressed in FAT-1 Mice
2.3. Anti-Viral CD8+ T Cell Responses in Peripheral Blood of FAT-1 Mice Were Suppressed
2.4. Anti-Viral CD8+ T Cell Responses in FAT-1 Mice Were Affected by Extracellular Factors
2.5. Anti-Viral CD4+ T Cell Responses in FAT-1 Mice Were Impaired
2.6. Anti-Viral CD8+ T Cells Carrying the Fat-1 Gene Exhibited a Decreased Capability to Proliferate and Produce Inflammatory Cytokines against LCMV Infection
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Virus and Infection
4.3. Reagents and Antibodies
4.4. Determination of LCMV Titers
4.5. Isolation of Splenocytes
4.6. Intracellular Cytokine Staining
4.7. Flow Cytometric Analysis
4.8. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Kang, K.W.; Kim, S.; Cho, Y.-B.; Ryu, S.R.; Seo, Y.-J.; Lee, S.-M. Endogenous n-3 Polyunsaturated Fatty Acids Are Beneficial to Dampen CD8+ T Cell-Mediated Inflammatory Response upon the Viral Infection in Mice. Int. J. Mol. Sci. 2019, 20, 4510. https://doi.org/10.3390/ijms20184510
Kang KW, Kim S, Cho Y-B, Ryu SR, Seo Y-J, Lee S-M. Endogenous n-3 Polyunsaturated Fatty Acids Are Beneficial to Dampen CD8+ T Cell-Mediated Inflammatory Response upon the Viral Infection in Mice. International Journal of Molecular Sciences. 2019; 20(18):4510. https://doi.org/10.3390/ijms20184510
Chicago/Turabian StyleKang, Kyung Won, Seyoung Kim, Yong-Bin Cho, Seung Rok Ryu, Young-Jin Seo, and Sang-Myeong Lee. 2019. "Endogenous n-3 Polyunsaturated Fatty Acids Are Beneficial to Dampen CD8+ T Cell-Mediated Inflammatory Response upon the Viral Infection in Mice" International Journal of Molecular Sciences 20, no. 18: 4510. https://doi.org/10.3390/ijms20184510