Reconstitution in Proteoliposomes of the Recombinant Human Riboflavin Transporter 2 (SLC52A2) Overexpressed in E. coli
Abstract
:1. Introduction
2. Results
2.1. Expression and Purification of Riboflavin Transporter 2 (RFVT2)
2.2. Characterization of the Transport Activity of RFVT2
2.3. Transport Assay of the Fibroblast Riboflavin Transporter
2.4. Homology Structural Model of RFVT2
3. Discussion
4. Materials and Methods
4.1. Overexpression
4.2. Purification
4.3. Reconstitution of RFVT2 into Liposomes
4.4. Transport Measurements
4.5. Other Methods
Author Contributions
Funding
Conflicts of Interest
Abbreviations
FMN | Riboflavin 5′-monophosphate |
FAD | Flavin adenine dinucleotide |
RFVT2 | Riboflavin Transporter 2 corresponding to SLC52A2 |
C12E8 | Octaethylene glycol monododecyl ether |
NEM | N-Ethylmaleimide |
PLP | Pyridoxal 5′-phosphate |
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Console, L.; Tolomeo, M.; Colella, M.; Barile, M.; Indiveri, C. Reconstitution in Proteoliposomes of the Recombinant Human Riboflavin Transporter 2 (SLC52A2) Overexpressed in E. coli. Int. J. Mol. Sci. 2019, 20, 4416. https://doi.org/10.3390/ijms20184416
Console L, Tolomeo M, Colella M, Barile M, Indiveri C. Reconstitution in Proteoliposomes of the Recombinant Human Riboflavin Transporter 2 (SLC52A2) Overexpressed in E. coli. International Journal of Molecular Sciences. 2019; 20(18):4416. https://doi.org/10.3390/ijms20184416
Chicago/Turabian StyleConsole, Lara, Maria Tolomeo, Matilde Colella, Maria Barile, and Cesare Indiveri. 2019. "Reconstitution in Proteoliposomes of the Recombinant Human Riboflavin Transporter 2 (SLC52A2) Overexpressed in E. coli" International Journal of Molecular Sciences 20, no. 18: 4416. https://doi.org/10.3390/ijms20184416