Next Article in Journal
DNA- and DNA-Protein-Crosslink Repair in Plants
Previous Article in Journal
The Mechanisms Involved in Morphine Addiction: An Overview
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 20
Issue 17
10.3390/ijms20174303
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Transcriptome Analysis Reveals Key Seed-Development Genes in Common Buckwheat (Fagopyrum esculentum)

by
Hongyou Li
1,*,
Qiuyu Lv
2,
Jiao Deng
1,
Juan Huang
1,
Fang Cai
1,
Chenggang Liang
1,
Qijiao Chen
1,
Yan Wang
1,
Liwei Zhu
1,
Xiaona Zhang
1 and
Qingfu Chen
1,*
1
Research Center of Buckwheat Industry Technology, Guizhou Normal University, Guiyang 550001, China
2
School of Big Data and Computer Science, Guizhou Normal University, Guiyang 550025, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(17), 4303; https://doi.org/10.3390/ijms20174303
Submission received: 8 July 2019 / Revised: 16 August 2019 / Accepted: 28 August 2019 / Published: 3 September 2019
(This article belongs to the Section Molecular Plant Sciences)
Browse Figures
Review Reports Versions Notes

Abstract

Seed development is an essential and complex process, which is involved in seed size change and various nutrients accumulation, and determines crop yield and quality. Common buckwheat (Fagopyrum esculentum Moench) is a widely cultivated minor crop with excellent economic and nutritional value in temperate zones. However, little is known about the molecular mechanisms of seed development in common buckwheat (Fagopyrum esculentum). In this study, we performed RNA-Seq to investigate the transcriptional dynamics and identify the key genes involved in common buckwheat seed development at three different developmental stages. A total of 4619 differentially expressed genes (DEGs) were identified. Based on the results of Gene Ontology (GO) and KEGG analysis of DEGs, many key genes involved in the seed development, including the Ca2+ signal transduction pathway, the hormone signal transduction pathways, transcription factors (TFs), and starch biosynthesis-related genes, were identified. More importantly, 18 DEGs were identified as the key candidate genes for seed size through homologous query using the known seed size-related genes from different seed plants. Furthermore, 15 DEGs from these identified as the key genes of seed development were selected to confirm the validity of the data by using quantitative real-time PCR (qRT-PCR), and the results show high consistency with the RNA-Seq results. Taken together, our results revealed the underlying molecular mechanisms of common buckwheat seed development and could provide valuable information for further studies, especially for common buckwheat seed improvement.
Keywords: common buckwheat; seed development; seed size; RNA-Seq; DEGs; transcription factors common buckwheat; seed development; seed size; RNA-Seq; DEGs; transcription factors

Share and Cite

MDPI and ACS Style

Li, H.; Lv, Q.; Deng, J.; Huang, J.; Cai, F.; Liang, C.; Chen, Q.; Wang, Y.; Zhu, L.; Zhang, X.; et al. Transcriptome Analysis Reveals Key Seed-Development Genes in Common Buckwheat (Fagopyrum esculentum). Int. J. Mol. Sci. 2019, 20, 4303. https://doi.org/10.3390/ijms20174303

AMA Style

Li H, Lv Q, Deng J, Huang J, Cai F, Liang C, Chen Q, Wang Y, Zhu L, Zhang X, et al. Transcriptome Analysis Reveals Key Seed-Development Genes in Common Buckwheat (Fagopyrum esculentum). International Journal of Molecular Sciences. 2019; 20(17):4303. https://doi.org/10.3390/ijms20174303

Chicago/Turabian Style

Li, Hongyou, Qiuyu Lv, Jiao Deng, Juan Huang, Fang Cai, Chenggang Liang, Qijiao Chen, Yan Wang, Liwei Zhu, Xiaona Zhang, and et al. 2019. "Transcriptome Analysis Reveals Key Seed-Development Genes in Common Buckwheat (Fagopyrum esculentum)" International Journal of Molecular Sciences 20, no. 17: 4303. https://doi.org/10.3390/ijms20174303

APA Style

Li, H., Lv, Q., Deng, J., Huang, J., Cai, F., Liang, C., Chen, Q., Wang, Y., Zhu, L., Zhang, X., & Chen, Q. (2019). Transcriptome Analysis Reveals Key Seed-Development Genes in Common Buckwheat (Fagopyrum esculentum). International Journal of Molecular Sciences, 20(17), 4303. https://doi.org/10.3390/ijms20174303

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop