Cucumber Mildew Resistance Locus O Interacts with Calmodulin and Regulates Plant Cell Death Associated with Plant Immunity
Abstract
:1. Introduction
2. Results
2.1. CsMLO-Mediated Regulation of CsCaM Expression Patterns in Cucumber Cotyledons
2.2. CsMLO1 Interacts with CsCaM3 in Yeast and Plants
2.3. Subcellular Colocalization of CsMLO1 and CsCaM3 in Plants
2.4. Inhibition of CsCaM3-Triggered Defense Response in CsMLO1-Overexpressing Cucumber Cotyledons
2.5. CsMLO1 Silencing Enhances Cucumber Resistance to C. cassiicola Infection
3. Discussion
4. Materials and methods
4.1. Plant Materials and Pathogen Inoculation
4.2. RNA Extraction, cDNA Synthesis, and Reverse-Transcription Quantitative PCR
4.3. Yeast Two-Hybrid Assay
4.4. Firefly Luciferase Complementation Imaging Assay and Western Blot
4.5. Bimolecular Fluorescence Complementation Analysis
4.6. Construction of Fusion Proteins, Plant Transformation, and Fluorescence Microscopy
4.7. Histochemical Analysis
4.8. Cucumber Cotyledon Transformation
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Material Name | Disease Index | Resistance |
---|---|---|
35S::GFP | 43.33 | S |
35S::CsMLO1 | 73.61 | HS |
35S::CsCaM3 | 21.48 | MR |
35S::CsMLO1/35S::CsCaM3 | 49.46 | S |
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Yu, G.; Wang, X.; Chen, Q.; Cui, N.; Yu, Y.; Fan, H. Cucumber Mildew Resistance Locus O Interacts with Calmodulin and Regulates Plant Cell Death Associated with Plant Immunity. Int. J. Mol. Sci. 2019, 20, 2995. https://doi.org/10.3390/ijms20122995
Yu G, Wang X, Chen Q, Cui N, Yu Y, Fan H. Cucumber Mildew Resistance Locus O Interacts with Calmodulin and Regulates Plant Cell Death Associated with Plant Immunity. International Journal of Molecular Sciences. 2019; 20(12):2995. https://doi.org/10.3390/ijms20122995
Chicago/Turabian StyleYu, Guangchao, Xiangyu Wang, Qiumin Chen, Na Cui, Yang Yu, and Haiyan Fan. 2019. "Cucumber Mildew Resistance Locus O Interacts with Calmodulin and Regulates Plant Cell Death Associated with Plant Immunity" International Journal of Molecular Sciences 20, no. 12: 2995. https://doi.org/10.3390/ijms20122995