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Int. J. Mol. Sci. 2018, 19(9), 2689; https://doi.org/10.3390/ijms19092689

Genome-Wide DNA Methylation Comparison between Brassica napus Genic Male Sterile Line and Restorer Line

1
National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, National Sub-Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan 430070, China
2
Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA
*
Author to whom correspondence should be addressed.
Received: 3 July 2018 / Revised: 30 August 2018 / Accepted: 4 September 2018 / Published: 10 September 2018
(This article belongs to the Section Molecular Plant Sciences)
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Abstract

DNA methylation is an essential epigenetic modification that dynamically regulates gene expression during plant development. However, few studies have determined the DNA methylation profiles of male-sterile rapeseed. Here, we conducted a global comparison of DNA methylation patterns between the rapeseed genic male sterile line 7365A and its near-isogenic fertile line 7365B by whole-genome bisulfite sequencing (WGBS). Profiling of the genome-wide DNA methylation showed that the methylation level in floral buds was lower than that in leaves and roots. Besides, a total of 410 differentially methylated region-associated genes (DMGs) were identified in 7365A relative to 7365B. Traditional bisulfite sequencing polymerase chain reaction (PCR) was performed to validate the WGBS data. Eleven DMGs were found to be involved in anther and pollen development, which were analyzed by quantitative PCR. In particular, Bnams4 was hypo-methylated in 7365A, and its expression was up-regulated, which might affect other DMGs and thus control the male sterility. This study provided genome-wide DNA methylation profiles of floral buds and important clues for revealing the molecular mechanism of genic male sterility in rapeseed. View Full-Text
Keywords: Brassica napus; genic male sterility; floral bud; whole-genome bisulfite sequencing Brassica napus; genic male sterility; floral bud; whole-genome bisulfite sequencing
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Wang, Z.; Wu, X.; Wu, Z.; An, H.; Yi, B.; Wen, J.; Ma, C.; Shen, J.; Fu, T.; Tu, J. Genome-Wide DNA Methylation Comparison between Brassica napus Genic Male Sterile Line and Restorer Line. Int. J. Mol. Sci. 2018, 19, 2689.

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