Next Article in Journal
RBM4a-SRSF3-MAP4K4 Splicing Cascade Constitutes a Molecular Mechanism for Regulating Brown Adipogenesis
Next Article in Special Issue
Crosstalk of the Brassinosteroid Signalosome with Phytohormonal and Stress Signaling Components Maintains a Balance between the Processes of Growth and Stress Tolerance
Previous Article in Journal
Myosin Head Configurations in Resting and Contracting Murine Skeletal Muscle
Previous Article in Special Issue
PpSARK Regulates Moss Senescence and Salt Tolerance through ABA Related Pathway
Article Menu
Issue 9 (September) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2018, 19(9), 2645; https://doi.org/10.3390/ijms19092645

Low Infection of Phelipanche aegyptiaca in Micro-Tom Mutants Deficient in CAROTENOID CLEAVAGE DIOXYGENASE 8

1
Graduate School of Life Sciences, Toyo University, Itakura-machi, Ora-gun, Gunma 374-0193, Japan
2
Department of Applied Biosciences, Toyo University, Itakura-machi, Ora-gun, Gunma 374-0193, Japan
3
Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan
4
Tsukuba Plant Innovation Research Center, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan
5
Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Naka-ku, Sakai, Osaka 599-8531, Japan
6
College of Life, Environment, and Advanced Sciences, Osaka Prefecture University, Naka-ku, Sakai, Osaka 599-8531, Japan
7
Graduate School of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, Japan
8
Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan
9
Graduate School of Life Sciences, Tohoku University, Katahira, Aoba-ku, Sendai 980-8577, Japan
*
Author to whom correspondence should be addressed.
Received: 8 August 2018 / Revised: 1 September 2018 / Accepted: 4 September 2018 / Published: 6 September 2018
Full-Text   |   PDF [3697 KB, uploaded 6 September 2018]   |  

Abstract

Strigolactones (SLs), a group of plant hormones, induce germination of root-parasitic plants and inhibit shoot branching in many plants. Shoot branching is an important trait that affects the number and quality of flowers and fruits. Root-parasitic plants, such as Phelipanche spp., infect tomato roots and cause economic damage in Europe and North Africa—hence why resistant tomato cultivars are needed. In this study, we found carotenoid cleavage dioxygenase 8-defective mutants of Micro-Tom tomato (slccd8) by the “targeting induced local lesions in genomes” (TILLING) method. The mutants showed excess branching, which was suppressed by exogenously applied SL. Grafting shoot scions of the slccd8 mutants onto wild-type (WT) rootstocks restored normal branching in the scions. The levels of endogenous orobanchol and solanacol in WT were enough detectable, whereas that in the slccd8 mutants were below the detection limit of quantification analysis. Accordingly, root exudates of the slccd8 mutants hardly stimulated seed germination of root parasitic plants. In addition, SL deficiency did not critically affect the fruit traits of Micro-Tom. Using a rhizotron system, we also found that Phelipanche aegyptiaca infection was lower in the slccd8 mutants than in wild-type Micro-Tom because of the low germination. We propose that the slccd8 mutants might be useful as new tomato lines resistant to P. aegyptiaca. View Full-Text
Keywords: carotenoid cleavage dioxygenase 8; Orobanche minor; Phelipanche aegyptiaca; shoot branching; Solanum lycopersicum; strigolactones carotenoid cleavage dioxygenase 8; Orobanche minor; Phelipanche aegyptiaca; shoot branching; Solanum lycopersicum; strigolactones
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Hasegawa, S.; Tsutsumi, T.; Fukushima, S.; Okabe, Y.; Saito, J.; Katayama, M.; Shindo, M.; Yamada, Y.; Shimomura, K.; Yoneyama, K.; Akiyama, K.; Aoki, K.; Ariizumi, T.; Ezura, H.; Yamaguchi, S.; Umehara, M. Low Infection of Phelipanche aegyptiaca in Micro-Tom Mutants Deficient in CAROTENOID CLEAVAGE DIOXYGENASE 8. Int. J. Mol. Sci. 2018, 19, 2645.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top