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Molecular Pathogenesis of Radiation-Induced Cell Toxicity in Stem Cells
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Int. J. Mol. Sci. 2018, 19(8), 2378; https://doi.org/10.3390/ijms19082378

Oridonin Enhances Radiation-Induced Cell Death by Promoting DNA Damage in Non-Small Cell Lung Cancer Cells

1
Division of Radiation Biomedical Research, Korea Institute of Radiological and Medical Sciences, Seoul 01812, Korea
2
K-Herb Research Center, Korea Institute of Oriental Medicine, Daejeon 34054, Korea
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 7 August 2018 / Accepted: 10 August 2018 / Published: 13 August 2018
(This article belongs to the Collection Radiation Toxicity in Cells)
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Abstract

Although many attempts have been made to improve the efficacy of radiotherapy to treat cancer, radiation resistance is still an obstacle in lung cancer treatment. Oridonin is a natural compound with promising antitumor efficacy that can trigger cancer cell death; however, its direct cellular targets, efficacy as a radiosensitizer, and underlying mechanisms of activity remain unclear. Herein, we report that oridonin exhibits additive cytotoxic and antitumor activity with radiation using the H460 non-small cell lung cancer cell lines. We assessed the effect of oridonin by proliferation, clonogenic, reactive oxygen species (ROS) production, DNA damage, and apoptosis assays. In vitro, oridonin enhanced the radiation-induced inhibition of cell growth and clonogenic survival. Oridonin also facilitated radiation-induced ROS production and DNA damage and enhanced apoptotic cell death. In vivo, the combination of oridonin and radiation effectively inhibited H460 xenograft tumor growth, with higher caspase-3 activation and H2A histone family member X (H2AX) phosphorylation compared with that of radiation alone. Our findings suggest that oridonin possesses a novel mechanism to enhance radiation therapeutic responses by increasing DNA damage and apoptosis. In conclusion, oridonin may be a novel small molecule to improve radiotherapy in non-small cell lung cancer. View Full-Text
Keywords: oridonin; radiation; sensitization; ROS; apoptosis oridonin; radiation; sensitization; ROS; apoptosis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Park, H.; Jeong, Y.J.; Han, N.-K.; Kim, J.S.; Lee, H.-J. Oridonin Enhances Radiation-Induced Cell Death by Promoting DNA Damage in Non-Small Cell Lung Cancer Cells. Int. J. Mol. Sci. 2018, 19, 2378.

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