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Int. J. Mol. Sci. 2018, 19(7), 1963; https://doi.org/10.3390/ijms19071963

Isolation of Adipose-Derived Stem/Stromal Cells from Cryopreserved Fat Tissue and Transplantation into Rats with Spinal Cord Injury

1
Department of Pharmacology, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki, Kanagawa 216-8511, Japan
2
Institute of Medical Science, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki, Kanagawa 216-8512, Japan
3
Institute for Animal Experimentation, St. Marianna University Graduate School of Medicine, 2-16-1 Sugao, Miyamae-ku, Kawasaki, Kanagawa 216-8511, Japan
*
Author to whom correspondence should be addressed.
Received: 30 May 2018 / Revised: 25 June 2018 / Accepted: 30 June 2018 / Published: 5 July 2018
(This article belongs to the Special Issue Adipose Stem Cells)
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Abstract

Adipose tissue contains multipotent cells known as adipose-derived stem/stromal cells (ASCs), which have therapeutic potential for various diseases. Although the demand for adipose tissue for research use remains high, no adipose tissue bank exists. In this study, we attempted to isolate ASCs from cryopreserved adipose tissue with the aim of developing a banking system. ASCs were isolated from fresh and cryopreserved adipose tissue of rats and compared for proliferation (doubling time), differentiation capability (adipocytes), and cytokine (hepatocyte growth factor and vascular endothelial growth factor) secretion. Finally, ASCs (2.5 × 106) were intravenously infused into rats with spinal cord injury, after which hindlimb motor function was evaluated. Isolation and culture of ASCs from cryopreserved adipose tissue were possible, and their characteristics were not significantly different from those of fresh tissue. Transplantation of ASCs derived from cryopreserved tissue significantly promoted restoration of hindlimb movement function in injured model rats. These results indicate that cryopreservation of adipose tissue may be an option for clinical application. View Full-Text
Keywords: adipose tissue; adipose-derived stem/stromal cells; cryopreservation; spinal cord injury; transplantation; tissue bank adipose tissue; adipose-derived stem/stromal cells; cryopreservation; spinal cord injury; transplantation; tissue bank
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Ohta, Y.; Takenaga, M.; Hamaguchi, A.; Ootaki, M.; Takeba, Y.; Kobayashi, T.; Watanabe, M.; Iiri, T.; Matsumoto, N. Isolation of Adipose-Derived Stem/Stromal Cells from Cryopreserved Fat Tissue and Transplantation into Rats with Spinal Cord Injury. Int. J. Mol. Sci. 2018, 19, 1963.

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