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Int. J. Mol. Sci. 2018, 19(7), 1952; https://doi.org/10.3390/ijms19071952

Application of Millifluidics to Encapsulate and Support Viable Human Mesenchymal Stem Cells in a Polysaccharide Hydrogel

1
Inserm, UMR 1229, RMeS, Regenerative Medicine and Skeleton, Université de Nantes, ONIRIS, F-44042 Nantes, France
2
CHU Nantes, Pharmacie Centrale, PHU 11, F-44093 Nantes, France
3
INRA UR1268, Biopolymères Interactions Assemblages, F-44300 Nantes, France
4
UFR Sciences Pharmaceutiques et Biologiques, Université de Nantes, F-44035 Nantes, France
5
CHU Nantes, PHU4 OTONN, F-44093 Nantes, France
6
UFR Odontologie, Université de Nantes, F-44042 Nantes, France
*
Author to whom correspondence should be addressed.
Received: 14 June 2018 / Revised: 28 June 2018 / Accepted: 29 June 2018 / Published: 3 July 2018
(This article belongs to the Special Issue Novel Biomaterials for Tissue Engineering 2018)
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Abstract

Human adipose-derived stromal cells (hASCs) are widely known for their immunomodulatory and anti-inflammatory properties. This study proposes a method to protect cells during and after their injection by encapsulation in a hydrogel using a droplet millifluidics technique. A biocompatible, self-hardening biomaterial composed of silanized-hydroxypropylmethylcellulose (Si-HPMC) hydrogel was used and dispersed in an oil continuous phase. Spherical particles with a mean diameter of 200 μm could be obtained in a reproducible manner. The viability of the encapsulated hASCs in the Si-HPMC particles was 70% after 14 days in vitro, confirming that the Si-HPMC particles supported the diffusion of nutrients, vitamins, and glucose essential for survival of the encapsulated hASCs. The combination of droplet millifluidics and biomaterials is therefore a very promising method for the development of new cellular microenvironments, with the potential for applications in biomedical engineering. View Full-Text
Keywords: droplet millifluidics; encapsulation; human adipose-derived stromal cells; hydrogel; self-hardening; silanized-hydroxypropylmethylcellulose; biomedical; degenerative disease droplet millifluidics; encapsulation; human adipose-derived stromal cells; hydrogel; self-hardening; silanized-hydroxypropylmethylcellulose; biomedical; degenerative disease
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Nativel, F.; Renard, D.; Hached, F.; Pinta, P.-G.; D’Arros, C.; Weiss, P.; Le Visage, C.; Guicheux, J.; Billon-Chabaud, A.; Grimandi, G. Application of Millifluidics to Encapsulate and Support Viable Human Mesenchymal Stem Cells in a Polysaccharide Hydrogel. Int. J. Mol. Sci. 2018, 19, 1952.

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