Detection of Dystrophin Dp71 in Human Skeletal Muscle Using an Automated Capillary Western Assay System
Abstract
:1. Introduction
2. Results
2.1. Detection of Dp71 mRNA in Human Skeletal Muscle
2.2. Detection of Over-Expressed Dp71 Protein by the Simple Western
2.3. Detection and Suppression of Dp71 in Rhabdomyosarcoma Cells
2.4. Detection of Dp71 Protein in Skeletal Muscles from Normal and DMDmdx Mice
2.5. Detection of Dp71 in Human Skeletal Muscle
3. Discussion
4. Materials and Methods
4.1. Cells
4.2. Muscle Samples
4.3. mRNA Analysis
4.4. Over-Expression of Dp71
4.5. Protein Sample Preparation
4.6. Simple Western Analysis
4.7. Antisense Oligonucleotide to Induce Exon 75 Skipping
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Kawaguchi, T.; Niba, E.T.E.; Rani, A.Q.M.; Onishi, Y.; Koizumi, M.; Awano, H.; Matsumoto, M.; Nagai, M.; Yoshida, S.; Sakakibara, S.; et al. Detection of Dystrophin Dp71 in Human Skeletal Muscle Using an Automated Capillary Western Assay System. Int. J. Mol. Sci. 2018, 19, 1546. https://doi.org/10.3390/ijms19061546
Kawaguchi T, Niba ETE, Rani AQM, Onishi Y, Koizumi M, Awano H, Matsumoto M, Nagai M, Yoshida S, Sakakibara S, et al. Detection of Dystrophin Dp71 in Human Skeletal Muscle Using an Automated Capillary Western Assay System. International Journal of Molecular Sciences. 2018; 19(6):1546. https://doi.org/10.3390/ijms19061546
Chicago/Turabian StyleKawaguchi, Tatsuya, Emma Tabe Eko Niba, Abdul Qawee Mahyoob Rani, Yoshiyuki Onishi, Makoto Koizumi, Hiroyuki Awano, Masaaki Matsumoto, Masashi Nagai, Shinobu Yoshida, Sachiko Sakakibara, and et al. 2018. "Detection of Dystrophin Dp71 in Human Skeletal Muscle Using an Automated Capillary Western Assay System" International Journal of Molecular Sciences 19, no. 6: 1546. https://doi.org/10.3390/ijms19061546