The Disordered C-Terminus of Yeast Hsf1 Contains a Cryptic Low-Complexity Amyloidogenic Region
Abstract
:1. Introduction
2. Results
2.1. Identification of a Cryptic Amyloid Sequence Inside a Disordered and Low Complexity Region in Hsf1
2.2. The Amyloid Core of Hsf1 Assembles into β-Sheet Enriched Aggregates
2.3. Hsf1 Amyloid Core Forms Amyloid-Like Fibrillary Structures
2.4. Hsf1 Amyloid Core Self-Assembles Very Fast
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Computational Identification of Hsf1 Soft Amyloid Cores in Low Complexity Regions
4.3. Hsf1 Soft Amyloid Core Peptide Preparation
4.4. Synchronous Light Scattering
4.5. Bis-ANS Binding
4.6. Attenuated Total Reflectance (ATR) Fourier Transform Infrared (FTIR) Spectroscopy
4.7. Binding to Amyloid Dyes
4.8. Transmission Electron Microscopy (TEM)
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Protein | Amyloid Core | Gravy Score |
---|---|---|
Hsf1 | INDIIFNTNLANNLSNYN | −0.283 |
ASYN | GVLYVG | 1.683 |
GGAVVTGVTAVAQ | 1.238 | |
Aβ42 | GAIIGLMVGGVVI | 2.462 |
QKLVFFAE | 0.562 |
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Pujols, J.; Santos, J.; Pallarès, I.; Ventura, S. The Disordered C-Terminus of Yeast Hsf1 Contains a Cryptic Low-Complexity Amyloidogenic Region. Int. J. Mol. Sci. 2018, 19, 1384. https://doi.org/10.3390/ijms19051384
Pujols J, Santos J, Pallarès I, Ventura S. The Disordered C-Terminus of Yeast Hsf1 Contains a Cryptic Low-Complexity Amyloidogenic Region. International Journal of Molecular Sciences. 2018; 19(5):1384. https://doi.org/10.3390/ijms19051384
Chicago/Turabian StylePujols, Jordi, Jaime Santos, Irantzu Pallarès, and Salvador Ventura. 2018. "The Disordered C-Terminus of Yeast Hsf1 Contains a Cryptic Low-Complexity Amyloidogenic Region" International Journal of Molecular Sciences 19, no. 5: 1384. https://doi.org/10.3390/ijms19051384