Calcium-Dependent Protein Kinase Family Genes Involved in Ethylene-Induced Natural Rubber Production in Different Hevea brasiliensis Cultivars
Abstract
:1. Introduction
2. Results
2.1. Identification and Characteristics of CPK Members in H. brasiliensis
2.2. Gene Structure, Duplication Events and Syntenic Analysis
2.3. Tissue-Specific and Ethylene-Induced Expression Analysis of HbCPKs in Three Rubber Tree Varieties
2.4. Cis-Element Distribution Analysis of Promoter Regions in HbCPKs
2.5. Protein Expression and PPI Analyses of Ethylene-Induced Proteins in Latex
3. Discussion
4. Materials and Methods
4.1. Identification and Sequence Analyses of CPK Gene Family in Rubber
4.2. Gene Structure, Duplication Event and Syntenic Analysis
4.3. Plant Material and Ethylene Treatment
4.4. Gene Expression Profiling of HbCPKs
4.5. Cis-Element Distribution Analysis in HbCPK Promoter Regions
4.6. Interaction Network Analysis of Ethylene-Induced Proteins in Latex
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Paralogous Genes | Ka | Ks | Ka/Ks | Selective Pressure |
---|---|---|---|---|
HbCPK16–HbCPK28 | 0.0129 | 0.1660 | 0.0777 | Purifying selection |
HbCPK1–HbCPK26 | 0.0276 | 0.2004 | 0.1377 | Purifying selection |
HbCPK3–HbCPK8 | 0.0193 | 0.2290 | 0.0843 | Purifying selection |
HbCPK13–HbCPK30 | 0.0274 | 0.1840 | 0.1489 | Purifying selection |
HbCPK4–HbCPK11 | 0.0308 | 0.2527 | 0.1219 | Purifying selection |
HbCPK9–HbCPK18 | 0.0434 | 0.1876 | 0.2313 | Purifying selection |
HbCPK2–HbCPK7 | 0.0227 | 0.1941 | 0.1170 | Purifying selection |
HbCPK21–HbCPK22 | 0.0169 | 0.2004 | 0.0843 | Purifying selection |
HbCPK19–HbCPK20 | 0.0214 | 0.1767 | 0.1211 | Purifying selection |
Testing Group | Mt b | M1 c | M2 d | Χ2 | P e |
---|---|---|---|---|---|
HbCPK4/HbCPK11 with MeCPK33-2 | 489 | 20 | 13 | 1.48 | 0.22302 |
HbCPK9/HbCPK18 with MeCPK3 | 457 | 7 | 47 | 29.63 | 0.00001 |
HbCPK16/HbCPK28 with MeCPK6-1 | 529 | 8 | 7 | 0.07 | 0.79625 |
HbCPK26/HbCPK1 with MeCPK20-1 | 250 | 5 | 11 | 2.25 | 0.13361 |
HbCPK3/HbCPK8 with MeCPK30 | 514 | 7 | 16 | 3.52 | 0.06057 |
HbCPK30/HbCPK13 with MeCPK32-2 | 485 | 10 | 17 | 1.81 | 0.17793 |
HbCPK19/HbCPK20 with MeCPK30 | 370 | 6 | 0 | 6.00 | 0.01431 |
HbCPK2/HbCPK7 with MeCPK17-2 | 465 | 16 | 9 | 1.96 | 0.16151 |
HbCPK22/HbCPK21 with MeCPK28-1 | 507 | 17 | 10 | 1.81 | 0.17793 |
HbCPK | Peptide | H2O-48 h | Eth-48 h | H2O-96 h | Eth-96 h |
---|---|---|---|---|---|
Numbers | (114) | (115) | (116) | (117) | |
HbCPK9 | 14 | 1.0000 * | 1.0471 | 1.0186 | 1.0965 |
HbCPK12 | 2 | 1.0000 | 3.3712 | 1.1478 | 3.1326 |
HbCPK16 | 6 | 1.0000 | 4.0926 | 2.1478 | 4.4463 |
HbCPK17 | 9 | 1.0000 | 9.7351 | 1.2985 | 9.1039 |
HbCPK18 | 7 | 1.0000 | 2.3335 | 0.8017 | 2.1677 |
HbCPK24 | 8 | 1.0000 | 13.9211 | 1.6346 | 12.5966 |
HbCPK28 | 6 | 1.0000 | 4.1687 | 1.7061 | 5.2481 |
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Zhu, L.; Jin, X.; Xie, Q.; Yao, Q.; Wang, X.; Li, H. Calcium-Dependent Protein Kinase Family Genes Involved in Ethylene-Induced Natural Rubber Production in Different Hevea brasiliensis Cultivars. Int. J. Mol. Sci. 2018, 19, 947. https://doi.org/10.3390/ijms19040947
Zhu L, Jin X, Xie Q, Yao Q, Wang X, Li H. Calcium-Dependent Protein Kinase Family Genes Involved in Ethylene-Induced Natural Rubber Production in Different Hevea brasiliensis Cultivars. International Journal of Molecular Sciences. 2018; 19(4):947. https://doi.org/10.3390/ijms19040947
Chicago/Turabian StyleZhu, Liping, Xiang Jin, Quanliang Xie, Qi Yao, Xuchu Wang, and Hongbin Li. 2018. "Calcium-Dependent Protein Kinase Family Genes Involved in Ethylene-Induced Natural Rubber Production in Different Hevea brasiliensis Cultivars" International Journal of Molecular Sciences 19, no. 4: 947. https://doi.org/10.3390/ijms19040947