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Int. J. Mol. Sci. 2018, 19(2), 597; https://doi.org/10.3390/ijms19020597

AOX1-Subfamily Gene Members in Olea europaea cv. “Galega Vulgar”—Gene Characterization and Expression of Transcripts during IBA-Induced in Vitro Adventitious Rooting

1
Departamento de Fitotecnia, ICAAM—Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Universidade de Évora, Pólo da Mitra, Ap. 94, 7006-554 Évora, Portugal
2
Institute of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Kraków, 31-120 Kraków, Poland
3
ITQB NOVA, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal
4
Functional Cell Reprogramming and Organism Plasticity (FunCrop), EU Marie Curie Chair, ICAAM, Universidade de Évora, 7006-554 Évora, Portugal
5
Functional Genomics and Bioinformatics, Department of Biochemistry and Molecular Biology, Federal University of Ceará, 60020-181Fortaleza, Brazil
6
Science and Technology Park Alentejo (PACT), 7005-841 Évora, Portugal
*
Authors to whom correspondence should be addressed.
Received: 21 November 2017 / Revised: 5 February 2018 / Accepted: 8 February 2018 / Published: 17 February 2018
(This article belongs to the Special Issue Plant Mitochondria)
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Abstract

Propagation of some Olea europaea L. cultivars is strongly limited due to recalcitrant behavior in adventitious root formation by semi-hardwood cuttings. One example is the cultivar ”Galega vulgar”. The formation of adventitious roots is considered a morphological response to stress. Alternative oxidase (AOX) is the terminal oxidase of the alternative pathway of the plant mitochondrial electron transport chain. This enzyme is well known to be induced in response to several biotic and abiotic stress situations. This work aimed to characterize the alternative oxidase 1 (AOX1)-subfamily in olive and to analyze the expression of transcripts during the indole-3-butyric acid (IBA)-induced in vitro adventitious rooting (AR) process. OeAOX1a (acc. no. MF410318) and OeAOX1d (acc. no. MF410319) were identified, as well as different transcript variants for both genes which resulted from alternative polyadenylation events. A correlation between transcript accumulation of both OeAOX1a and OeAOX1d transcripts and the three distinct phases (induction, initiation, and expression) of the AR process in olive was observed. Olive AOX1 genes seem to be associated with the induction and development of adventitious roots in IBA-treated explants. A better understanding of the molecular mechanisms underlying the stimulus needed for the induction of adventitious roots may help to develop more targeted and effective rooting induction protocols in order to improve the rooting ability of difficult-to-root cultivars. View Full-Text
Keywords: vegetative propagation; olive; adventitious rooting; auxins; IBA; plant mitochondria; alternative oxidase; alternative polyadenylation; transposable elements; gene expression vegetative propagation; olive; adventitious rooting; auxins; IBA; plant mitochondria; alternative oxidase; alternative polyadenylation; transposable elements; gene expression
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Velada, I.; Grzebelus, D.; Lousa, D.; M. Soares, C.; Santos Macedo, E.; Peixe, A.; Arnholdt-Schmitt, B.; G. Cardoso, H. AOX1-Subfamily Gene Members in Olea europaea cv. “Galega Vulgar”—Gene Characterization and Expression of Transcripts during IBA-Induced in Vitro Adventitious Rooting. Int. J. Mol. Sci. 2018, 19, 597.

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