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Int. J. Mol. Sci. 2018, 19(2), 592; https://doi.org/10.3390/ijms19020592

A Biochemical Approach to Detect Oxidative Stress in Infertile Women Undergoing Assisted Reproductive Technology Procedures

1
Department of Experimental and Clinical Biomedical Sciences “Mario Serio”, University of Florence, Viale Morgagni 50, 50134 Florence, Italy
2
Department of Information Engineering and Mathematics, University of Siena, 53100 Siena, Italy
3
Assisted Reproduction Center, Careggi University Hospital, 50134 Florence, Italy
4
Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 2 January 2018 / Revised: 12 February 2018 / Accepted: 13 February 2018 / Published: 16 February 2018
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
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Abstract

Oxidative stress plays a major role in critical biological processes in human reproduction. However, a reliable and biologically accurate indicator of this condition does not yet exist. On these bases, the aim of this study was to assess and compare the blood and follicular fluid (FF) redox status of 45 infertile subjects (and 45 age-matched controls) undergoing in vitro fertilization (IVF), and explore possible relationships between the assessed redox parameters and IVF outcomes. Reactive Oxygen Species (ROS) production, assessed by flow cytometry analysis in blood leukocytes and granulosa cells, significantly increased (p < 0.05) in infertile patients. Also, oxidative stress markers—ThioBarbituric Acid-Reactive Substances (TBARS) as an index of lipid peroxidation, and Oxygen Radical Absorbance Capacity (ORAC) to account for total antioxidant capacity, both assayed by fluorometric procedures—in blood and FF were significantly (p < 0.001) modified in infertile patients compared to the control group. Moreover, a significant correlation between blood redox markers and FF redox markers was evident. An ORAC/TBARS ratio, defined as the redox index (RI), was obtained in the plasma and FF of the patients and controls. In the patients, the plasma RI was about 3.4-fold (p < 0.0001) lower than the control, and the FF RI was about six-fold (p < 0.0001) lower than the control. Interestingly, both the plasma RI and FF RI results were significantly correlated (p < 0.05) to the considered outcome parameters (metaphase II, fertilization rate, and ongoing pregnancies). Given the reported findings, a strict monitoring of redox parameters in assisted reproductive techniques and infertility management is recommended. View Full-Text
Keywords: oxidative stress; in vitro fertilization (IVF); infertile women; follicular fluid (FF); granulosa cells oxidative stress; in vitro fertilization (IVF); infertile women; follicular fluid (FF); granulosa cells
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Becatti, M.; Fucci, R.; Mannucci, A.; Barygina, V.; Mugnaini, M.; Criscuoli, L.; Giachini, C.; Bertocci, F.; Picone, R.; Emmi, G.; Evangelisti, P.; Rizzello, F.; Cozzi, C.; Taddei, N.; Fiorillo, C.; Coccia, M.E. A Biochemical Approach to Detect Oxidative Stress in Infertile Women Undergoing Assisted Reproductive Technology Procedures. Int. J. Mol. Sci. 2018, 19, 592.

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