Physicochemical Properties of the Mammalian Molecular Chaperone HSP60
AbstractThe E. coli GroEL/GroES chaperonin complex acts as a folding cage by producing a bullet-like asymmetric complex, and GroEL exists as double rings regardless of the presence of adenosine triphosphate (ATP). Its mammalian chaperonin homolog, heat shock protein, HSP60, and co-chaperonin, HSP10, play an essential role in protein folding by capturing unfolded proteins in the HSP60/HSP10 complex. However, the structural transition in ATPase-dependent reaction cycle has remained unclear. We found nucleotide-dependent association and dissociation of the HSP60/HSP10 complex using various analytical techniques under near physiological conditions. Our results showed that HSP60 exist as a significant number of double-ring complexes (football- and bullet-type complexes) and a small number of single-ring complexes in the presence of ATP and HSP10. HSP10 binds to HSP60 in the presence of ATP, which increased the HSP60 double-ring formation. After ATP is hydrolyzed to Adenosine diphosphate (ADP), HSP60 released the HSP10 and the dissociation of the double-ring to single-rings occurred. These results indicated that HSP60/HSP10 undergoes an ATP-dependent transition between the single- and double-rings in their system that is highly distinctive from the GroEL/GroES system particularly in the manner of complex formation and the roles of ATP binding and hydrolysis in the reaction cycle. View Full-Text
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Ishida, R.; Okamoto, T.; Motojima, F.; Kubota, H.; Takahashi, H.; Tanabe, M.; Oka, T.; Kitamura, A.; Kinjo, M.; Yoshida, M.; Otaka, M.; Grave, E.; Itoh, H. Physicochemical Properties of the Mammalian Molecular Chaperone HSP60. Int. J. Mol. Sci. 2018, 19, 489.
Ishida R, Okamoto T, Motojima F, Kubota H, Takahashi H, Tanabe M, Oka T, Kitamura A, Kinjo M, Yoshida M, Otaka M, Grave E, Itoh H. Physicochemical Properties of the Mammalian Molecular Chaperone HSP60. International Journal of Molecular Sciences. 2018; 19(2):489.Chicago/Turabian Style
Ishida, Ryuichi; Okamoto, Tomoya; Motojima, Fumihiro; Kubota, Hiroshi; Takahashi, Hiroki; Tanabe, Masako; Oka, Toshihiko; Kitamura, Akira; Kinjo, Masataka; Yoshida, Masasuke; Otaka, Michiro; Grave, Ewa; Itoh, Hideaki. 2018. "Physicochemical Properties of the Mammalian Molecular Chaperone HSP60." Int. J. Mol. Sci. 19, no. 2: 489.
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