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Int. J. Mol. Sci. 2018, 19(2), 452; https://doi.org/10.3390/ijms19020452

The Application of Non-Invasive Apoptosis Detection Sensor (NIADS) on Histone Deacetylation Inhibitor (HDACi)-Induced Breast Cancer Cell Death

1
Research Center for Tumor Medical Science, China Medical University, Taichung 40402, Taiwan
2
Graduate Institutes of New Drug Development, China Medical University, Taichung 40402, Taiwan
3
Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
4
Division of General Surgery, Department of Surgery, Shuang Ho Hospital, Taipei Medical University, New Taipei City 23561,Taiwan
5
Institute of Bioinformatics and Systems Biology, National Chiao Tung University, Hsinchu 30068, Taiwan
6
Bioinformatics Center, Institute for Chemical Research, Kyoto University, Kyoto 611-0011, Japan
7
Department of Endocrinology and metabolism, Cathay General Hospital, Taipei 10630, Taiwan
8
Department of Laboratory Medicine, Shuang Ho Hospital, Taipei Medical University, Taipei 23561, Taiwan
9
Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
10
Division of Colorectal Surgery, Department of Surgery, Wan Fang Hospital, Taipei Medical University, Taipei 11696, Taiwan
11
Division of Colorectal Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei Medical University, Taipei 11031, Taiwan
12
Cancer Research Center and Translational Laboratory, Department of Medical Research, Taipei Medical University Hospital, Taipei Medical University, Taipei 11031, Taiwan
13
Graduate Institute of Cancer Biology and Drug Discovery, Taipei Medical University, Taipei 11031, Taiwan
14
Comprehensive Breast Cancer Center, Changhua Christian Hospital, Changhua 50006, Taiwan
15
School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan
16
Ph.D. Program in Medicine Biotechnology, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
17
Comprehensive Cancer Center of Taipei Medical University, Taipei 11031, Taiwan
*
Authors to whom correspondence should be addressed.
Received: 1 December 2017 / Revised: 22 January 2018 / Accepted: 26 January 2018 / Published: 2 February 2018
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Abstract

Breast cancer is the most common malignancy in women and the second leading cause of cancer death in women. Triple negative breast cancer (TNBC) subtype is a breast cancer subset without ER (estrogen receptor), PR (progesterone receptor) and HER2 (human epidermal growth factor receptor 2) expression, limiting treatment options and presenting a poorer survival rate. Thus, we investigated whether histone deacetylation inhibitor (HDACi) could be used as potential anti-cancer therapy on breast cancer cells. In this study, we found TNBC and HER2-enriched breast cancers are extremely sensitive to Panobinostat, Belinostat of HDACi via experiments of cell viability assay, apoptotic marker identification and flow cytometry measurement. On the other hand, we developed a bioluminescence-based live cell non-invasive apoptosis detection sensor (NIADS) detection system to evaluate the quantitative and kinetic analyses of apoptotic cell death by HDAC treatment on breast cancer cells. In addition, the use of HDACi may also contribute a synergic anti-cancer effect with co-treatment of chemotherapeutic agent such as doxorubicin on TNBC cells (MDA-MB-231), but not in breast normal epithelia cells (MCF-10A), providing therapeutic benefits against breast tumor in the clinic. View Full-Text
Keywords: live cell non-invasive apoptosis detection sensor (NIADS); triple negative breast cancer (TNBC); HDACi live cell non-invasive apoptosis detection sensor (NIADS); triple negative breast cancer (TNBC); HDACi
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Hsu, K.-W.; Huang, C.-Y.; Tam, K.-W.; Lin, C.-Y.; Huang, L.-C.; Lin, C.-L.; Hsieh, W.-S.; Chi, W.-M.; Chang, Y.-J.; Wei, P.-L.; Chen, S.-T.; Lee, C.-H. The Application of Non-Invasive Apoptosis Detection Sensor (NIADS) on Histone Deacetylation Inhibitor (HDACi)-Induced Breast Cancer Cell Death. Int. J. Mol. Sci. 2018, 19, 452.

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