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Int. J. Mol. Sci. 2018, 19(2), 359; doi:10.3390/ijms19020359

Aging Donor-Derived Human Mesenchymal Stem Cells Exhibit Reduced Reactive Oxygen Species Loads and Increased Differentiation Potential Following Serial Expansion on a PEG-PCL Copolymer Substrate

1
Department of Biomedical Engineering, Vanderbilt University, Nashville, TN 37235, USA
2
Department of Materials and Department of Bioengineering, Imperial College London, London SW7 2AZ, UK
3
Severance Biomedical Science Institute, College of Medicine, Yonsei University, Seoul 03722, Korea
4
Department of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA
5
Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, Korea
6
Department of Otorhinolaryngology, College of Medicine, Yonsei University, Seoul 03722, Korea
*
Author to whom correspondence should be addressed.
Received: 9 January 2018 / Revised: 22 January 2018 / Accepted: 23 January 2018 / Published: 25 January 2018
(This article belongs to the Special Issue Novel Biomaterials for Tissue Engineering 2018)
View Full-Text   |   Download PDF [3986 KB, uploaded 25 January 2018]   |  

Abstract

Human mesenchymal stem cells (hMSCs) have been widely studied for therapeutic development in tissue engineering and regenerative medicine. They can be harvested from human donors via tissue biopsies, such as bone marrow aspiration, and cultured to reach clinically relevant cell numbers. However, an unmet issue lies in the fact that the hMSC donors for regenerative therapies are more likely to be of advanced age. Their stem cells are not as potent compared to those of young donors, and continue to lose healthy, stemness-related activities when the hMSCs are serially passaged in tissue culture plates. Here, we have developed a cheap, scalable, and effective copolymer film to culture hMSCs obtained from aged human donors over several passages without loss of reactive oxygen species (ROS) handling or differentiation capacity. Assays of cell morphology, reactive oxygen species load, and differentiation potential demonstrate the effectiveness of copolymer culture on reduction in senescence-related activities of aging donor-derived hMSCs that could hinder the therapeutic potential of autologous stem cell therapies. View Full-Text
Keywords: biomaterial; copolymer; stem cell; regenerative medicine; cell culture biomaterial; copolymer; stem cell; regenerative medicine; cell culture
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Balikov, D.A.; Crowder, S.W.; Lee, J.B.; Lee, Y.; Ko, U.H.; Kang, M.-L.; Kim, W.S.; Shin, J.H.; Sung, H.-J. Aging Donor-Derived Human Mesenchymal Stem Cells Exhibit Reduced Reactive Oxygen Species Loads and Increased Differentiation Potential Following Serial Expansion on a PEG-PCL Copolymer Substrate. Int. J. Mol. Sci. 2018, 19, 359.

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