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Int. J. Mol. Sci. 2018, 19(2), 350; doi:10.3390/ijms19020350

Comparative Digital Gene Expression Analysis of Tissue-Cultured Plantlets of Highly Resistant and Susceptible Banana Cultivars in Response to Fusarium oxysporum

1
College of Horticulture, South China Agricultural University, Guangzhou 510642, China
2
Institute of Biotechnology, Guangxi Academy of Agricultural Sciences, Nanning 530007, China
3
Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Cell Biology, Faculty of Science, Palacký University, 783 01 Olomouc, Czech Republic
*
Author to whom correspondence should be addressed.
Received: 8 December 2017 / Revised: 15 January 2018 / Accepted: 16 January 2018 / Published: 24 January 2018
(This article belongs to the Section Molecular Botany)
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Abstract

Banana Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc) is one of the most destructive soil-borne diseases. In this study, young tissue-cultured plantlets of banana (Musa spp. AAA) cultivars differing in Foc susceptibility were used to reveal their differential responses to this pathogen using digital gene expression (DGE). Data were evaluated by various bioinformatic tools (Venn diagrams, gene ontology (GO) annotation and Kyoto encyclopedia of genes and genomes (KEGG) pathway analyses) and immunofluorescence labelling method to support the identification of gene candidates determining the resistance of banana against Foc. Interestingly, we have identified MaWRKY50 as an important gene involved in both constitutive and induced resistance. We also identified new genes involved in the resistance of banana to Foc, including several other transcription factors (TFs), pathogenesis-related (PR) genes and some genes related to the plant cell wall biosynthesis or degradation (e.g., pectinesterases, β-glucosidases, xyloglucan endotransglucosylase/hydrolase and endoglucanase). The resistant banana cultivar shows activation of PR-3 and PR-4 genes as well as formation of different constitutive cell barriers to restrict spreading of the pathogen. These data suggest new mechanisms of banana resistance to Foc. View Full-Text
Keywords: banana Fusarium wilt; DGE; Venn diagram; GO annotation; KEGG pathways; resistance genes banana Fusarium wilt; DGE; Venn diagram; GO annotation; KEGG pathways; resistance genes
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Niu, Y.; Hu, B.; Li, X.; Chen, H.; Takáč, T.; Šamaj, J.; Xu, C. Comparative Digital Gene Expression Analysis of Tissue-Cultured Plantlets of Highly Resistant and Susceptible Banana Cultivars in Response to Fusarium oxysporum. Int. J. Mol. Sci. 2018, 19, 350.

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