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Int. J. Mol. Sci. 2018, 19(2), 324; https://doi.org/10.3390/ijms19020324

Identification of Proteins Involved in Carbohydrate Metabolism and Energy Metabolism Pathways and Their Regulation of Cytoplasmic Male Sterility in Wheat

College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi, China
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Received: 19 December 2017 / Revised: 13 January 2018 / Accepted: 21 January 2018 / Published: 23 January 2018
(This article belongs to the Section Molecular Plant Sciences)
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Abstract

Cytoplasmic male sterility (CMS) where no functional pollen is produced has important roles in wheat breeding. The anther is a unique organ for male gametogenesis and its abnormal development can cause male sterility. However, the mechanisms and regulatory networks related to plant male sterility are poorly understood. In this study, we conducted comparative analyses using isobaric tags for relative and absolute quantification (iTRAQ) of the pollen proteins in a CMS line and its wheat maintainer. Differentially abundant proteins (DAPs) were analyzed based on Gene Ontology classifications, metabolic pathways and transcriptional regulation networks using Blast2GO. We identified 5570 proteins based on 23,277 peptides, which matched with 73,688 spectra, including proteins in key pathways such as glyceraldehyde-3-phosphate dehydrogenase, pyruvate kinase and 6-phosphofructokinase 1 in the glycolysis pathway, isocitrate dehydrogenase and citrate synthase in the tricarboxylic acid cycle and nicotinamide adenine dinucleotide (NADH)-dehydrogenase and adenosine-triphosphate (ATP) synthases in the oxidative phosphorylation pathway. These proteins may comprise a network that regulates male sterility in wheat. Quantitative real time polymerase chain reaction (qRT-PCR) analysis, ATP assays and total sugar assays validated the iTRAQ results. These DAPs could be associated with abnormal pollen grain formation and male sterility. Our findings provide insights into the molecular mechanism related to male sterility in wheat. View Full-Text
Keywords: anther proteomics; cytoplasmic male sterility; hybrid wheat; isobaric tags for relative and absolute quantification (iTRAQ) anther proteomics; cytoplasmic male sterility; hybrid wheat; isobaric tags for relative and absolute quantification (iTRAQ)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Geng, X.; Ye, J.; Yang, X.; Li, S.; Zhang, L.; Song, X. Identification of Proteins Involved in Carbohydrate Metabolism and Energy Metabolism Pathways and Their Regulation of Cytoplasmic Male Sterility in Wheat. Int. J. Mol. Sci. 2018, 19, 324.

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