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Int. J. Mol. Sci. 2018, 19(1), 309; https://doi.org/10.3390/ijms19010309

Enhancing Flower Color through Simultaneous Expression of the B-peru and mPAP1 Transcription Factors under Control of a Flower-Specific Promoter

1
National Institute of Agricultural Science, Rural Development Administration, Jeonju 54874, Korea
2
Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Korea
These authors contributed to work equally.
*
Author to whom correspondence should be addressed.
Received: 19 December 2017 / Revised: 16 January 2018 / Accepted: 17 January 2018 / Published: 20 January 2018
(This article belongs to the Section Molecular Plant Sciences)
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Abstract

Flower color is a main target for flower breeding. A transgenic approach for flower color modification requires a transgene and a flower-specific promoter. Here, we expressed the B-peru gene encoding a basic helix loop helix (bHLH) transcription factor (TF) together with the mPAP1 gene encoding an R2R3 MYB TF to enhance flower color in tobacco (Nicotiana tabacum L.), using the tobacco anthocyanidin synthase (ANS) promoter (PANS) to drive flower-specific expression. The transgenic tobacco plants grew normally and produced either dark pink (PANSBP_DP) or dark red (PANSBP_DR) flowers. Quantitative real time polymerase chain reaction (qPCR) revealed that the expression of five structural genes in the flavonoid biosynthetic pathway increased significantly in both PANSBP_DP and PANSBP_DR lines, compared with the non-transformed (NT) control. Interestingly, the expression of two regulatory genes constituting the active MYB-bHLH-WD40 repeat (WDR) (MBW) complex decreased significantly in the PANSBP_DR plants but not in the PANSBP_DP plants. Total flavonol and anthocyanin abundance correlated with flower color, with an increase of 1.6–43.2 fold in the PANSBP_DP plants and 2.0–124.2 fold in the PANSBP_DR plants. Our results indicate that combinatorial expression of B-peru and mPAP1 genes under control of the ANS promoter can be a useful strategy for intensifying flower color without growth retardation. View Full-Text
Keywords: anthocyanin; flower color; flower-specific promoter; transcription factor; tobacco anthocyanin; flower color; flower-specific promoter; transcription factor; tobacco
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Kim, D.-H.; Park, S.; Lee, J.-Y.; Ha, S.-H.; Lim, S.-H. Enhancing Flower Color through Simultaneous Expression of the B-peru and mPAP1 Transcription Factors under Control of a Flower-Specific Promoter. Int. J. Mol. Sci. 2018, 19, 309.

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