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Int. J. Mol. Sci. 2018, 19(1), 188; https://doi.org/10.3390/ijms19010188

Carbon Monoxide Potentiates High Temperature-Induced Nicotine Biosynthesis in Tobacco

1
Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing 210037, China
2
College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China
3
Laboratory of Tobacco Agriculture, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China
4
Key Laboratory of Forest Genetics and Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China
5
Research Center of Saline and Alkali Land of State Forestry Administration, China Academy of Forestry, Beijing 100091, China
*
Author to whom correspondence should be addressed.
Received: 9 November 2017 / Revised: 19 December 2017 / Accepted: 29 December 2017 / Published: 8 January 2018
(This article belongs to the Section Molecular Plant Sciences)
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Abstract

Carbon monoxide (CO) acts as an important signal in many physiological responses in plants, but its role in plant secondary metabolism is still unknown. Nicotine is the main alkaloid generated in tobacco and the plant hormone jasmonic acid (JA) has previously been reported to efficiently induce its biosynthesis. Whether and how CO interacts with JA to regulate nicotine biosynthesis in tobacco remains elusive. In this study, we demonstrate that high temperature (HT) induces quick accumulation of nicotine in tobacco roots, combined with an increase in CO and JA concentration. Suppressing CO generation reduced both JA and nicotine biosynthesis, whereas exogenous application of CO increased JA and nicotine content. CO causes an increased expression of NtPMT1 (a key nicotine biosynthesis enzyme), via promoting NtMYC2a binding to the G-box region of its promoter, leading to heightened nicotine levels under HT conditions. These data suggest a novel function for CO in stimulating nicotine biosynthesis in tobacco under HT stress, through a JA signal. View Full-Text
Keywords: carbon monoxide; high temperature; nicotine; jasmonic acid; tobacco carbon monoxide; high temperature; nicotine; jasmonic acid; tobacco
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Cheng, T.; Hu, L.; Wang, P.; Yang, X.; Peng, Y.; Lu, Y.; Chen, J.; Shi, J. Carbon Monoxide Potentiates High Temperature-Induced Nicotine Biosynthesis in Tobacco. Int. J. Mol. Sci. 2018, 19, 188.

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