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Int. J. Mol. Sci. 2018, 19(1), 155; https://doi.org/10.3390/ijms19010155

LHCSR Expression under HSP70/RBCS2 Promoter as a Strategy to Increase Productivity in Microalgae

Dipartimento di Biotecnologie, Università di Verona, Strada Le Grazie 15, 37134 Verona, Italy
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Received: 7 December 2017 / Revised: 24 December 2017 / Accepted: 31 December 2017 / Published: 5 January 2018
(This article belongs to the Special Issue Photosynthesis)
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Abstract

Microalgae are unicellular photosynthetic organisms considered as potential alternative sources for biomass, biofuels or high value products. However, limited biomass productivity is commonly experienced in their cultivating system despite their high potential. One of the reasons for this limitation is the high thermal dissipation of the light absorbed by the outer layers of the cultures exposed to high light caused by the activation of a photoprotective mechanism called non-photochemical quenching (NPQ). In the model organism for green algae Chlamydomonas reinhardtii, NPQ is triggered by pigment binding proteins called light-harvesting-complexes-stress-related (LHCSRs), which are over-accumulated in high light. It was recently reported that biomass productivity can be increased both in microalgae and higher plants by properly tuning NPQ induction. In this work increased light use efficiency is reported by introducing in C. reinhardtii a LHCSR3 gene under the control of Heat Shock Protein 70/RUBISCO small chain 2 promoter in a npq4 lhcsr1 background, a mutant strain knockout for all LHCSR genes. This complementation strategy leads to a low expression of LHCSR3, causing a strong reduction of NPQ induction but is still capable of protecting from photodamage at high irradiance, resulting in an improved photosynthetic efficiency and higher biomass accumulation. View Full-Text
Keywords: photosynthesis; photoprotection; microalgae; plant biotechnology; light harvesting; non-photochemical quenching photosynthesis; photoprotection; microalgae; plant biotechnology; light harvesting; non-photochemical quenching
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Perozeni, F.; Stella, G.R.; Ballottari, M. LHCSR Expression under HSP70/RBCS2 Promoter as a Strategy to Increase Productivity in Microalgae. Int. J. Mol. Sci. 2018, 19, 155.

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