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Int. J. Mol. Sci. 2017, 18(9), 2001; doi:10.3390/ijms18092001

The Impact of Non-Lethal Single-Dose Radiation on Tumor Invasion and Cytoskeletal Properties

1
Institute of Anatomy and Cell Biology, Martin Luther University Halle-Wittenberg, Grosse Steinstrasse 52, 06108 Halle, Germany
2
Department of Radiation Oncology, Martin Luther University Halle-Wittenberg, Ernst-Grube-Strasse 40, 06120 Halle, Germany
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 15 August 2017 / Revised: 5 September 2017 / Accepted: 15 September 2017 / Published: 18 September 2017
(This article belongs to the Special Issue Glioma Cell Invasion)
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Abstract

Irradiation is the standard therapy for glioblastoma multiforme. Glioblastoma are highly resistant to radiotherapy and the underlying mechanisms remain unclear. To better understand the biological effects of irradiation on glioblastoma cells, we tested whether nonlethal irradiation influences the invasiveness, cell stiffness, and actin cytoskeleton properties. Two different glioblastoma cell lines were irradiated with 2 Gy and changes in mechanical and migratory properties and alterations in the actin structure were measured. The invasiveness of cell lines was determined using a co-culture model with organotypic hippocampal slice cultures. Irradiation led to changes in motility and a less invasive phenotype in both investigated cell lines that were associated with an increase in a ”generalized stiffness” and changes in the actin structure. In this study we demonstrate that irradiation can induce changes in the actin cytoskeleton and motility, which probably results in reduced invasiveness of glioblastoma cell lines. Furthermore, “generalized stiffness” was shown to be a profound marker of the invasiveness of a tumor cell population in our model. View Full-Text
Keywords: glioblastoma; cell mechanics; slice cultures; actin; phalloidin; radiation; cytoskeleton glioblastoma; cell mechanics; slice cultures; actin; phalloidin; radiation; cytoskeleton
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MDPI and ACS Style

Hohmann, T.; Grabiec, U.; Vogel, C.; Ghadban, C.; Ensminger, S.; Bache, M.; Vordermark, D.; Dehghani, F. The Impact of Non-Lethal Single-Dose Radiation on Tumor Invasion and Cytoskeletal Properties. Int. J. Mol. Sci. 2017, 18, 2001.

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