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Int. J. Mol. Sci. 2017, 18(9), 1950; doi:10.3390/ijms18091950

De Novo Assembly and Analysis of Polygonatum sibiricum Transcriptome and Identification of Genes Involved in Polysaccharide Biosynthesis

1
National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, Xi’an 710119, Shaanxi, China
2
College of Chemistry, Biology and Materials Science, East China University of Technology, Nanchang 330013, Jiangxi, China
3
College of Life Sciences and Food Engineering, Shaanxi XueQian Normal University, Xi’an 710119, Shaanxi, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 17 July 2017 / Revised: 7 September 2017 / Accepted: 8 September 2017 / Published: 12 September 2017
(This article belongs to the Special Issue Molecular Recognition of Carbohydrates)
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Abstract

Polygonatum sibiricum polysaccharides (PSPs) are used to improve immunity, alleviate dryness, promote the secretion of fluids, and quench thirst. However, the PSP biosynthetic pathway is largely unknown. Understanding the genetic background will help delineate that pathway at the molecular level so that researchers can develop better conservation strategies. After comparing the PSP contents among several different P. sibiricum germplasms, we selected two groups with the largest contrasts in contents and subjected them to HiSeq2500 transcriptome sequencing to identify the candidate genes involved in PSP biosynthesis. In all, 20 kinds of enzyme-encoding genes were related to PSP biosynthesis. The polysaccharide content was positively correlated with the expression patterns of β-fructofuranosidase (sacA), fructokinase (scrK), UDP-glucose 4-epimerase (GALE), Mannose-1-phosphate guanylyltransferase (GMPP), and UDP-glucose 6-dehydrogenase (UGDH), but negatively correlated with the expression of Hexokinase (HK). Through qRT-PCR validation and comprehensive analysis, we determined that sacA, HK, and GMPP are key genes for enzymes within the PSP metabolic pathway in P. sibiricum. Our results provide a public transcriptome dataset for this species and an outline of pathways for the production of polysaccharides in medicinal plants. They also present more information about the PSP biosynthesis pathway at the molecular level in P. sibiricum and lay the foundation for subsequent research of gene functions. View Full-Text
Keywords: Polygonatum sibiricum; polysaccharide; transcriptome; polysaccharide biosynthesis; qRT-PCR Polygonatum sibiricum; polysaccharide; transcriptome; polysaccharide biosynthesis; qRT-PCR
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Wang, S.; Wang, B.; Hua, W.; Niu, J.; Dang, K.; Qiang, Y.; Wang, Z. De Novo Assembly and Analysis of Polygonatum sibiricum Transcriptome and Identification of Genes Involved in Polysaccharide Biosynthesis. Int. J. Mol. Sci. 2017, 18, 1950.

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