Inhibition of NAT10 Suppresses Melanogenesis and Melanoma Growth by Attenuating Microphthalmia-Associated Transcription Factor (MITF) Expression
Abstract
:1. Introduction
2. Results
2.1. Remodelin Attenuates Melanin Synthesis by Repressing MITF (Microphthalmia-Associated Transcription Factor)
2.2. Genetic Silencing of NAT10 Suppresses Melanin Synthesis
2.3. Inhibition of NAT10 Delays Cell Cycle Progression via S-Phase Arrest
2.4. Inhibition of NAT10 Delays Melanoma Growth In Vitro and In Vivo
3. Discussion
4. Materials and Methods
4.1. Reagents and Antibodies
4.2. Cell Culture and Generation of Stable Cell Lines
4.3. Western Blotting
4.4. Determination of Melanin Contents
4.5. RNA Isolation and Quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR)
4.6. Tumor Xenograft Assay
4.7. Cellular Tyrosinase Activity Assay
4.8. Measurement of Cell Viability and Clonogenic Assay
4.9. Cell Cycle and Proliferation Assay
4.10. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
α-MSH | Alpha-melanocyte stimulating hormone |
NAT10 | N-acetyltransferase 10 |
MITF | Microphthalmia-associated transcription factor |
GNAT | GCN5-related N-acetyltransferases |
BCL2 | B-cell lymphoma 2 |
DCT | Dopachrome tautomerase |
UV | Ultraviolet |
HGPS | Hutchinson-Gilford progeria syndrome |
l-DOPA | l-3,4-dihydroxyphenylalanine |
CREB | cAMP response element-binding protein |
PAX3 | Paired box 3 |
SOX10 | SRY-related HMG-box 10 |
LEF-1 | Lymphoid enhancing factor-1 |
UBF | Upstream binding factor |
HAT | Histone acetyltransferase |
CDK2 | Cyclin-dependent kinase 2 |
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Oh, T.-I.; Lee, Y.-M.; Lim, B.-O.; Lim, J.-H. Inhibition of NAT10 Suppresses Melanogenesis and Melanoma Growth by Attenuating Microphthalmia-Associated Transcription Factor (MITF) Expression. Int. J. Mol. Sci. 2017, 18, 1924. https://doi.org/10.3390/ijms18091924
Oh T-I, Lee Y-M, Lim B-O, Lim J-H. Inhibition of NAT10 Suppresses Melanogenesis and Melanoma Growth by Attenuating Microphthalmia-Associated Transcription Factor (MITF) Expression. International Journal of Molecular Sciences. 2017; 18(9):1924. https://doi.org/10.3390/ijms18091924
Chicago/Turabian StyleOh, Taek-In, Yoon-Mi Lee, Beong-Ou Lim, and Ji-Hong Lim. 2017. "Inhibition of NAT10 Suppresses Melanogenesis and Melanoma Growth by Attenuating Microphthalmia-Associated Transcription Factor (MITF) Expression" International Journal of Molecular Sciences 18, no. 9: 1924. https://doi.org/10.3390/ijms18091924