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Int. J. Mol. Sci. 2017, 18(9), 1874; doi:10.3390/ijms18091874

Transgenic Cotton Plants Expressing the HaHR3 Gene Conferred Enhanced Resistance to Helicoverpa armigera and Improved Cotton Yield

1
The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China
2
Cotton Science Research Institute of Hunan Province, Changde 415101, Hunan, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 23 July 2017 / Revised: 15 August 2017 / Accepted: 26 August 2017 / Published: 30 August 2017
(This article belongs to the Section Molecular Botany)
View Full-Text   |   Download PDF [2515 KB, uploaded 30 August 2017]   |  

Abstract

RNA interference (RNAi) has been developed as an efficient technology. RNAi insect-resistant transgenic plants expressing double-stranded RNA (dsRNA) that is ingested into insects to silence target genes can affect the viability of these pests or even lead to their death. HaHR3, a molt-regulating transcription factor gene, was previously selected as a target expressed in bacteria and tobacco plants to control Helicoverpa armigera by RNAi technology. In this work, we selected the dsRNA-HaHR3 fragment to silence HaHR3 in cotton bollworm for plant mediated-RNAi research. A total of 19 transgenic cotton lines expressing HaHR3 were successfully cultivated, and seven generated lines were used to perform feeding bioassays. Transgenic cotton plants expressing dsHaHR3 were shown to induce high larval mortality and deformities of pupation and adult eclosion when used to feed the newly hatched larvae, and 3rd and 5th instar larvae of H. armigera. Moreover, HaHR3 transgenic cotton also demonstrated an improved cotton yield when compared with controls. View Full-Text
Keywords: RNA interference; plant mediated-RNAi; Helicoverpa armigera; HaHR3; transgenic cotton plants RNA interference; plant mediated-RNAi; Helicoverpa armigera; HaHR3; transgenic cotton plants
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MDPI and ACS Style

Han, Q.; Wang, Z.; He, Y.; Xiong, Y.; Lv, S.; Li, S.; Zhang, Z.; Qiu, D.; Zeng, H. Transgenic Cotton Plants Expressing the HaHR3 Gene Conferred Enhanced Resistance to Helicoverpa armigera and Improved Cotton Yield. Int. J. Mol. Sci. 2017, 18, 1874.

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