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Int. J. Mol. Sci. 2017, 18(7), 1579; doi:10.3390/ijms18071579

Characterization of the Dioscorin Gene Family in Dioscorea alata Reveals a Role in Tuber Development and Environmental Response

1
Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China
2
School of Biological Sciences and Technology, Liupanshui Normal University, Liupanshui 553001, China
3
Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China
4
Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 6 June 2017 / Revised: 16 July 2017 / Accepted: 17 July 2017 / Published: 20 July 2017
(This article belongs to the Section Molecular Botany)
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Abstract

Dioscorin is one of the major soluble proteins in yam tubers. Unlike other well-known plant storage proteins, such as patatin and sporamin, dioscorin is argued for its function as storage proteins, and the molecular mechanisms underlying its expressional complexity are little understood. In this study, we isolated five dioscorin genes from Dioscorea alata L., comprising three class A (Da-dio1, -3 and -4) and two class B (Da-dio2 and -5) isoforms. Expressions of all dioscorin genes gradually decreased in mother tubers during yam sprouting and regrowth. On the other hand, all dioscorin genes accumulated transcripts progressively with tuber development in new tubers, with Da-dio5 being the most prominent isoform. In yam leaves, the expressions of Da-dio5 were up-regulated by the treatments of five phytohormones (gibberellic acid, salicylic acid, indole-3-acetic acid, abscisic acid, and ethylene), and three abiotic stresses (high-temperature, low-temperature and drought). To further elucidate the regulatory mechanisms of Da-dio5 expressions, transgenic Arabidopsis plants harboring the Da-dio5 promoter-β-glucuronidase (GUS) fusion were generated. GUS staining showed that expressions of the Da-dio5 promoter were detected mainly in the shoot apical meristem (SAM) and hypocotyls, and enhanced by the treatments of the five hormones, and the three abiotic stresses mentioned above. These results suggest diverse roles of Da-dio5 in yam sprouting, regrowth, and tuberization, as well as in response to enviromental cues. View Full-Text
Keywords: dioscorin; cloning and expression; tuber development; promoter; Dioscorea alata L. dioscorin; cloning and expression; tuber development; promoter; Dioscorea alata L.
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MDPI and ACS Style

Liu, L.; Huang, Y.; Huang, X.; Yang, J.; Wu, W.; Xu, Y.; Cong, Z.; Xie, J.; Xia, W.; Huang, D. Characterization of the Dioscorin Gene Family in Dioscorea alata Reveals a Role in Tuber Development and Environmental Response. Int. J. Mol. Sci. 2017, 18, 1579.

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