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Int. J. Mol. Sci. 2017, 18(7), 1473; doi:10.3390/ijms18071473

Fluorogenic Labeling Strategies for Biological Imaging

1
École Normale Supérieure, PSL Research University, UPMC Univ Paris 06, CNRS, Département de Chimie, PASTEUR, 24 rue Lhomond, 75005 Paris, France
2
Sorbonne Universités, UPMC Univ Paris 06, ENS, CNRS, PASTEUR, 75005 Paris, France
*
Author to whom correspondence should be addressed.
Received: 21 June 2017 / Revised: 3 July 2017 / Accepted: 6 July 2017 / Published: 9 July 2017
(This article belongs to the Special Issue Fluorescent Proteins)
View Full-Text   |   Download PDF [1572 KB, uploaded 9 July 2017]   |  

Abstract

The spatiotemporal fluorescence imaging of biological processes requires effective tools to label intracellular biomolecules in living systems. This review presents a brief overview of recent labeling strategies that permits one to make protein and RNA strongly fluorescent using synthetic fluorogenic probes. Genetically encoded tags selectively binding the exogenously applied molecules ensure high labeling selectivity, while high imaging contrast is achieved using fluorogenic chromophores that are fluorescent only when bound to their cognate tag, and are otherwise dark. Beyond avoiding the need for removal of unbound synthetic dyes, these approaches allow the development of sophisticated imaging assays, and open exciting prospects for advanced imaging, particularly for multiplexed imaging and super-resolution microscopy. View Full-Text
Keywords: fluorogenic probes; protein labeling; RNA labeling fluorogenic probes; protein labeling; RNA labeling
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Li, C.; Tebo, A.G.; Gautier, A. Fluorogenic Labeling Strategies for Biological Imaging. Int. J. Mol. Sci. 2017, 18, 1473.

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