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Int. J. Mol. Sci. 2017, 18(7), 1360; doi:10.3390/ijms18071360

The Co-Expression Pattern of p63 and HDAC1: A Potential Way to Disclose Stem Cells in Interfollicular Epidermis

1
Department of Dermatology, Seoul National University Bundang Hospital, 166 Gumi-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-707, Korea
2
Department of Dermatology, Keimyung University School of Medicine, 56 Dalseong-Ro, Jung-Gu, Daegu 41931, Korea
3
P&G Group, Kobe 658-0032, Japan
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 1 June 2017 / Revised: 16 June 2017 / Accepted: 21 June 2017 / Published: 26 June 2017
(This article belongs to the Special Issue Molecular Research of Epidermal Stem Cells 2017)
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Abstract

Stem cell markers of interfollicular epidermis (IEF) have not been established thus far. The aim of this study is to suggest a new way to disclose IFE-stem cells by combining the expression of histone deacetylases (HDAC) 1 and p63. Immunohistochemical staining of HDAC1 and p63 was performed in six normal human samples. Moreover, a skin equivalent (SE) model was treated with suberoylanilohydroxamic acid (SAHA, an HDAC inhibitor) to elucidate the role of HDAC1. Finally, rapidly adhering (RA) keratinocytes to a type IV collagen, which have been identified to represent epidermal stem cells, were subjected to Western blot analysis with antibodies against HDAC1. In normal samples, there was a minor subpopulation comprised of p63-positive and HDAC1-negative cells in the basal layers. The proportion of this subpopulation was decreased with age. In the SE model, SAHA treatment increased the epidermal thickness and number of p63-positive cells in a dose dependent manner. After SAHA treatment, the expression of differentiation markers was decreased, while that of basement membrane markers was increased. In a Western blot analysis, HDAC1 was not expressed in RA cells. In conclusion, the combination of p63-positive and HDAC1-negative expressions can be a potential new way for distinguishing epidermal stem cells. View Full-Text
Keywords: epidermal stem cell; HDAC1; interfollicular epidermis; stem cell niche; p63; suberoylanilohydroxamic acid (SAHA) epidermal stem cell; HDAC1; interfollicular epidermis; stem cell niche; p63; suberoylanilohydroxamic acid (SAHA)
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Shin, J.-W.; Choi, H.-R.; Nam, K.-M.; Lee, H.-S.; Kim, S.-A.; Joe, H.-J.; Kazumi, T.; Park, K.-C. The Co-Expression Pattern of p63 and HDAC1: A Potential Way to Disclose Stem Cells in Interfollicular Epidermis. Int. J. Mol. Sci. 2017, 18, 1360.

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