The Co-Expression Pattern of p63 and HDAC1: A Potential Way to Disclose Stem Cells in Interfollicular Epidermis
AbstractStem cell markers of interfollicular epidermis (IEF) have not been established thus far. The aim of this study is to suggest a new way to disclose IFE-stem cells by combining the expression of histone deacetylases (HDAC) 1 and p63. Immunohistochemical staining of HDAC1 and p63 was performed in six normal human samples. Moreover, a skin equivalent (SE) model was treated with suberoylanilohydroxamic acid (SAHA, an HDAC inhibitor) to elucidate the role of HDAC1. Finally, rapidly adhering (RA) keratinocytes to a type IV collagen, which have been identified to represent epidermal stem cells, were subjected to Western blot analysis with antibodies against HDAC1. In normal samples, there was a minor subpopulation comprised of p63-positive and HDAC1-negative cells in the basal layers. The proportion of this subpopulation was decreased with age. In the SE model, SAHA treatment increased the epidermal thickness and number of p63-positive cells in a dose dependent manner. After SAHA treatment, the expression of differentiation markers was decreased, while that of basement membrane markers was increased. In a Western blot analysis, HDAC1 was not expressed in RA cells. In conclusion, the combination of p63-positive and HDAC1-negative expressions can be a potential new way for distinguishing epidermal stem cells. View Full-Text
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Shin, J.-W.; Choi, H.-R.; Nam, K.-M.; Lee, H.-S.; Kim, S.-A.; Joe, H.-J.; Kazumi, T.; Park, K.-C. The Co-Expression Pattern of p63 and HDAC1: A Potential Way to Disclose Stem Cells in Interfollicular Epidermis. Int. J. Mol. Sci. 2017, 18, 1360.
Shin J-W, Choi H-R, Nam K-M, Lee H-S, Kim S-A, Joe H-J, Kazumi T, Park K-C. The Co-Expression Pattern of p63 and HDAC1: A Potential Way to Disclose Stem Cells in Interfollicular Epidermis. International Journal of Molecular Sciences. 2017; 18(7):1360.Chicago/Turabian Style
Shin, Jung-Won; Choi, Hye-Ryung; Nam, Kyung-Mi; Lee, Hyun-Sun; Kim, Sung-Ae; Joe, Hyun-Jae; Kazumi, Toyama; Park, Kyoung-Chan. 2017. "The Co-Expression Pattern of p63 and HDAC1: A Potential Way to Disclose Stem Cells in Interfollicular Epidermis." Int. J. Mol. Sci. 18, no. 7: 1360.
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