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Int. J. Mol. Sci. 2017, 18(4), 807; doi:10.3390/ijms18040807

High Glucose Accelerates Cell Proliferation and Increases the Secretion and mRNA Expression of Osteopontin in Human Pancreatic Duct Epithelial Cells

1
Department of Gastroenterology, Faculty of Medicine, Yamagata University, Yamagata 990-9585, Japan
2
Hyogo Prefectural Nishinomiya Hospital, Nishinomiya 662-0918, Japan
*
Author to whom correspondence should be addressed.
Academic Editors: Srikumar Chellappan and Jaya Padmanabhan
Received: 25 February 2017 / Revised: 5 April 2017 / Accepted: 8 April 2017 / Published: 12 April 2017
(This article belongs to the Special Issue Pancreatic Disorders)
View Full-Text   |   Download PDF [1576 KB, uploaded 12 April 2017]   |  

Abstract

Background: The incidence of pancreatic cancer is increasing year-by-year in Japan. Among the diseases that complicate pancreatic cancer, diabetes is the most common. Recently, it has become evident that patients suffering from diabetes and obesity show increased expression of osteopontin (OPN). The purpose of this study was to investigate the effect of high glucose and high insulin culture conditions on a human pancreatic duct epithelial cell line (HPDE-6), focusing particularly on OPN expression. Methods: HPDE-6 were cultured under various conditions, employing several combinations of glucose (normal, 6 mM high, 30 mM, and 60 mM) and insulin (0.1 nM, 1 nM) concentration. Results: HPDE-6 cell proliferation was significantly accelerated under high glucose culture conditions in comparison to samples in 6 mM glucose, and was more prominent under high insulin conditions. At the same time, the expression of OPN mRNA was also increased significantly. In comparison with 6 mM glucose, the expression of 8-OHdG DNA was increased in high glucose culture. Conclusion: HPDE-6 cells show accelerated proliferation and increased OPN expression when cultured under high glucose and high insulin conditions. Furthermore, the cells show increased oxidative stress in the presence of high glucose. View Full-Text
Keywords: human pancreatic duct epithelial cell line (HPDE-6); osteopontin; diabetes; hyperinsulinemia; pancreatic ductal adenocarcinoma human pancreatic duct epithelial cell line (HPDE-6); osteopontin; diabetes; hyperinsulinemia; pancreatic ductal adenocarcinoma
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MDPI and ACS Style

Ito, M.; Makino, N.; Matsuda, A.; Ikeda, Y.; Kakizaki, Y.; Saito, Y.; Ueno, Y.; Kawata, S. High Glucose Accelerates Cell Proliferation and Increases the Secretion and mRNA Expression of Osteopontin in Human Pancreatic Duct Epithelial Cells. Int. J. Mol. Sci. 2017, 18, 807.

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