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Int. J. Mol. Sci. 2017, 18(4), 695; doi:10.3390/ijms18040695

An Amylase-Like Protein, AmyD, Is the Major Negative Regulator for α-Glucan Synthesis in Aspergillus nidulans during the Asexual Life Cycle

1
Key Laboratory of Molecular Epigenetics, Ministry of Education, Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, Jilin, China
2
Jilin Institute of Biology, Changchun 130012, Jilin, China
3
Department of Biology, University of Saskatchewan, 112 Science Place, Saskatoon, SK S7N 5E2, Canada
*
Author to whom correspondence should be addressed.
Academic Editor: Vaclav Vetvicka
Received: 11 February 2017 / Revised: 18 March 2017 / Accepted: 22 March 2017 / Published: 27 March 2017
(This article belongs to the Special Issue Glucan: New Perspectives on Biochemistry and Application)
View Full-Text   |   Download PDF [6273 KB, uploaded 27 March 2017]   |  

Abstract

α-Glucan affects fungal cell–cell interactions and is important for the virulence of pathogenic fungi. Interfering with production of α-glucan could help to prevent fungal infection. In our previous study, we reported that an amylase-like protein, AmyD, could repress α-glucan accumulation in Aspergillus nidulans. However, the underlying molecular mechanism was not clear. Here, we examined the localization of AmyD and found it was a membrane-associated protein. We studied AmyD function in α-glucan degradation, as well as with other predicted amylase-like proteins and three annotated α-glucanases. AmyC and AmyE share a substantial sequence identity with AmyD, however, neither affects α-glucan synthesis. In contrast, AgnB and MutA (but not AgnE) are functional α-glucanases that also repress α-glucan accumulation. Nevertheless, the functions of AmyD and these glucanases were independent from each other. The dynamics of α-glucan accumulation showed different patterns between the AmyD overexpression strain and the α-glucanase overexpression strains, suggesting AmyD may not be involved in the α-glucan degradation process. These results suggest the function of AmyD is to directly suppress α-glucan synthesis, but not to facilitate its degradation. View Full-Text
Keywords: AmyD; α-glucan; A. nidulans; cell wall; fungal virulence AmyD; α-glucan; A. nidulans; cell wall; fungal virulence
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He, X.; Li, S.; Kaminskyj, S. An Amylase-Like Protein, AmyD, Is the Major Negative Regulator for α-Glucan Synthesis in Aspergillus nidulans during the Asexual Life Cycle. Int. J. Mol. Sci. 2017, 18, 695.

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