Next Article in Journal
Hyper-Methylated Loci Persisting from Sessile Serrated Polyps to Serrated Cancers
Next Article in Special Issue
Pharmacological Modulation of Radiation Damage. Does It Exist a Chance for Other Substances than Hematopoietic Growth Factors and Cytokines?
Previous Article in Journal
Exosomes: From Garbage Bins to Promising Therapeutic Targets
Previous Article in Special Issue
Development of Orally Administered γ-Tocotrienol (GT3) Nanoemulsion for Radioprotection
Article Menu
Issue 3 (March) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2017, 18(3), 541; doi:10.3390/ijms18030541

Pre-Exposure Gene Expression in Baboons with and without Pancytopenia after Radiation Exposure

1
Bundeswehr Institute of Radiobiology, 80937 Munich, Germany
2
Institut de Recherche Biomedicale des Armees, 91220 Bretigny-sur-Orge, France
*
Author to whom correspondence should be addressed.
Academic Editor: Terrence Piva
Received: 27 January 2017 / Revised: 17 February 2017 / Accepted: 24 February 2017 / Published: 2 March 2017
(This article belongs to the Collection Radiation Toxicity in Cells)
View Full-Text   |   Download PDF [1247 KB, uploaded 2 March 2017]   |  

Abstract

Radiosensitivity differs in humans and likely among primates. The reasons are not well known. We examined pre-exposure gene expression in baboons (n = 17) who developed haematologic acute radiation syndrome (HARS) without pancytopenia or a more aggravated HARS with pancytopenia after irradiation. We evaluated gene expression in a two stage study design where stage I comprised a whole genome screen for messenger RNAs (mRNA) (microarray) and detection of 667 microRNAs (miRNA) (real-time quantitative polymerase chain reaction (qRT-PCR) platform). Twenty candidate mRNAs and nine miRNAs were selected for validation in stage II (qRT-PCR). None of the mRNA species could be confirmed during the validation step, but six of the nine selected candidate miRNA remained significantly different during validation. In particular, miR-425-5p (receiver operating characteristic = 0.98; p = 0.0003) showed nearly complete discrimination between HARS groups with and without pancytopenia. Target gene searches of miR-425-5p identified new potential mRNAs and associated biological processes linked with radiosensitivity. We found that one miRNA species examined in pre-exposure blood samples was associated with HARS characterized by pancytopenia and identified new target mRNAs that might reflect differences in radiosensitivity of irradiated normal tissue. View Full-Text
Keywords: radiosensitivity; pancytopenia; miRNA; gene expression; haematologic acute radiation syndrome (HARS) radiosensitivity; pancytopenia; miRNA; gene expression; haematologic acute radiation syndrome (HARS)
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Port, M.; Hérodin, F.; Valente, M.; Drouet, M.; Ullmann, R.; Majewski, M.; Abend, M. Pre-Exposure Gene Expression in Baboons with and without Pancytopenia after Radiation Exposure. Int. J. Mol. Sci. 2017, 18, 541.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top