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Int. J. Mol. Sci. 2017, 18(12), 2584; https://doi.org/10.3390/ijms18122584

In Vivo Imaging of Prostate Cancer Tumors and Metastasis Using Non-Specific Fluorescent Nanoparticles in Mice

1
Imagerie Moléculaire et Thérapies Innovantes en Oncologie, IMOTION, EA 7435, Bordeaux University, F33076 Bordeaux, France
2
CEA Grenoble, LETI-DTBS, MINATEC Campus, F38054 Grenoble, France
*
Authors to whom correspondence should be addressed.
Received: 18 October 2017 / Revised: 27 November 2017 / Accepted: 29 November 2017 / Published: 1 December 2017
(This article belongs to the Special Issue Chemical and Molecular Approach to Tumor Metastases)
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Abstract

With the growing interest in the use of nanoparticles (NPs) in nanomedicine, there is a crucial need for imaging and targeted therapies to determine NP distribution in the body after systemic administration, and to achieve strong accumulation in tumors with low background in other tissues. Accumulation of NPs in tumors results from different mechanisms, and appears extremely heterogeneous in mice models and rather limited in humans. Developing new tumor models in mice, with their low spontaneous NP accumulation, is thus necessary for screening imaging probes and for testing new targeting strategies. In the present work, accumulation of LipImageTM 815, a non-specific nanosized fluorescent imaging agent, was compared in subcutaneous, orthotopic and metastatic tumors of RM1 cells (murine prostate cancer cell line) by in vivo and ex vivo fluorescence imaging techniques. LipImageTM 815 mainly accumulated in liver at 24 h but also in orthotopic tumors. Limited accumulation occurred in subcutaneous tumors, and very low fluorescence was detected in metastasis. Altogether, these different tumor models in mice offered a wide range of NP accumulation levels, and a panel of in vivo models that may be useful to further challenge NP targeting properties. View Full-Text
Keywords: prostate cancer; fluorescence imaging; bioluminescence imaging; fluorescence tomography; enhanced permeability and retention (EPR) effect; LipImageTM prostate cancer; fluorescence imaging; bioluminescence imaging; fluorescence tomography; enhanced permeability and retention (EPR) effect; LipImageTM
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Genevois, C.; Hocquelet, A.; Mazzocco, C.; Rustique, E.; Couillaud, F.; Grenier, N. In Vivo Imaging of Prostate Cancer Tumors and Metastasis Using Non-Specific Fluorescent Nanoparticles in Mice. Int. J. Mol. Sci. 2017, 18, 2584.

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