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Int. J. Mol. Sci. 2016, 17(9), 1492; doi:10.3390/ijms17091492

Identifying Virulence-Associated Genes Using Transcriptomic and Proteomic Association Analyses of the Plant Parasitic Nematode Bursaphelenchus mucronatus

1
Collaborative Innovation Center of Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China
2
Institute of Forest Protection, College of Forestry, Nanjing Forestry University, Nanjing 210037, China
3
General Station of Forest Pest Management, The State Forestry Administration, Shenyang 110034, China
*
Author to whom correspondence should be addressed.
Academic Editor: Jianhua Zhu
Received: 19 June 2016 / Revised: 5 August 2016 / Accepted: 23 August 2016 / Published: 7 September 2016
(This article belongs to the Section Molecular Botany)
View Full-Text   |   Download PDF [5133 KB, uploaded 7 September 2016]   |  

Abstract

Bursaphelenchus mucronatus (B. mucronatus) isolates that originate from different regions may vary in their virulence, but their virulence-associated genes and proteins are poorly understood. Thus, we conducted an integrated study coupling RNA-Seq and isobaric tags for relative and absolute quantitation (iTRAQ) to analyse transcriptomic and proteomic data of highly and weakly virulent B. mucronatus isolates during the pathogenic processes. Approximately 40,000 annotated unigenes and 5000 proteins were gained from the isolates. When we matched all of the proteins with their detected transcripts, a low correlation coefficient of r = 0.138 was found, indicating probable post-transcriptional gene regulation involved in the pathogenic processes. A functional analysis showed that five differentially expressed proteins which were all highly expressed in the highly virulent isolate were involved in the pathogenic processes of nematodes. Peroxiredoxin, fatty acid- and retinol-binding protein, and glutathione peroxidase relate to resistance against plant defence responses, while β-1,4-endoglucanase and expansin are associated with the breakdown of plant cell walls. Thus, the pathogenesis of B. mucronatus depends on its successful survival in host plants. Our work adds to the understanding of B. mucronatus’ pathogenesis, and will aid in controlling B. mucronatus and other pinewood nematode species complexes in the future. View Full-Text
Keywords: Bursaphelenchus mucronatus; virulence-associated gene; transcriptomic; proteomic Bursaphelenchus mucronatus; virulence-associated gene; transcriptomic; proteomic
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Zhou, L.; Chen, F.; Pan, H.; Ye, J.; Dong, X.; Li, C.; Lin, F. Identifying Virulence-Associated Genes Using Transcriptomic and Proteomic Association Analyses of the Plant Parasitic Nematode Bursaphelenchus mucronatus. Int. J. Mol. Sci. 2016, 17, 1492.

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