Characterizing Aciniform Silk Repetitive Domain Backbone Dynamics and Hydrodynamic Modularity
AbstractSpider aciniform (wrapping) silk is a remarkable fibrillar biomaterial with outstanding mechanical properties. It is a modular protein consisting, in Argiope trifasciata, of a core repetitive domain of 200 amino acid units (W units). In solution, the W units comprise a globular folded core, with five α-helices, and disordered tails that are linked to form a ~63-residue intrinsically disordered linker in concatemers. Herein, we present nuclear magnetic resonance (NMR) spectroscopy-based 15N spin relaxation analysis, allowing characterization of backbone dynamics as a function of residue on the ps–ns timescale in the context of the single W unit (W1) and the two unit concatemer (W2). Unambiguous mapping of backbone dynamics throughout W2 was made possible by segmental NMR active isotope-enrichment through split intein-mediated trans-splicing. Spectral density mapping for W1 and W2 reveals a striking disparity in dynamics between the folded core and the disordered linker and tail regions. These data are also consistent with rotational diffusion behaviour where each globular domain tumbles almost independently of its neighbour. At a localized level, helix 5 exhibits elevated high frequency dynamics relative to the proximal helix 4, supporting a model of fibrillogenesis where this helix unfolds as part of the transition to a mixed α-helix/β-sheet fibre. View Full-Text
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Tremblay, M.-L.; Xu, L.; Sarker, M.; Liu, X.-Q.; Rainey, J.K. Characterizing Aciniform Silk Repetitive Domain Backbone Dynamics and Hydrodynamic Modularity. Int. J. Mol. Sci. 2016, 17, 1305.
Tremblay M-L, Xu L, Sarker M, Liu X-Q, Rainey JK. Characterizing Aciniform Silk Repetitive Domain Backbone Dynamics and Hydrodynamic Modularity. International Journal of Molecular Sciences. 2016; 17(8):1305.Chicago/Turabian Style
Tremblay, Marie-Laurence; Xu, Lingling; Sarker, Muzaddid; Liu, Xiang-Qin; Rainey, Jan K. 2016. "Characterizing Aciniform Silk Repetitive Domain Backbone Dynamics and Hydrodynamic Modularity." Int. J. Mol. Sci. 17, no. 8: 1305.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.