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Int. J. Mol. Sci. 2016, 17(8), 1264; doi:10.3390/ijms17081264

Droplet Digital PCR Based Androgen Receptor Variant 7 (AR-V7) Detection from Prostate Cancer Patient Blood Biopsies

1
Centre for Circulating Tumor Cell Diagnostics and Research, Ingham Institute for Applied Medical Research, 1 Campbell St., Liverpool, NSW 2170, Australia
2
South Western Clinical School, University of New South Wales, Goulburn St., Liverpool, NSW 2170, Australia
3
Western Sydney University Clinical School, Elizabeth St., Liverpool, NSW 2170, Australia
4
Department of Medical Oncology, Liverpool Hospital, Elizabeth St & Goulburn St., Liverpool, NSW 2170, Australia
*
Author to whom correspondence should be addressed.
Academic Editor: Dario Marchetti
Received: 30 June 2016 / Revised: 25 July 2016 / Accepted: 27 July 2016 / Published: 4 August 2016
(This article belongs to the Special Issue Circulating Tumor Cells)
View Full-Text   |   Download PDF [1606 KB, uploaded 4 August 2016]   |  

Abstract

Androgen receptor splice variant V7 (AR-V7) was recently identified as a valuable predictive biomarker in metastatic castrate-resistant prostate cancer. Here, we report a new, sensitive and accurate screen for AR-V7 mRNA expression directly from circulating tumor cells (CTCs): We combined EpCAM-based immunomagnetic CTC isolation using the IsoFlux microfluidic platform with droplet digital polymerase chain reaction (ddPCR) to analyze total AR and AR-V7 expression from prostate cancer patients CTCs. We demonstrate that AR-V7 is reliably detectable in enriched CTC samples with as little as five CTCs, even considering tumor heterogeneity, and confirm detection of AR-V7 in CTC samples from advanced prostate cancer (PCa) patients with AR-V7 detection limited to castrate resistant disease status in our sample set. Sensitive molecular analyses of circulating tumor cells (CTCs) or circulating tumor nucleic acids present exciting strategies to detect biomarkers, such as AR-V7 from non-invasive blood samples, so-called blood biopsies. View Full-Text
Keywords: biomarker; androgen receptor; AR-V7; prostate cancer; ddPCR; CTC biomarker; androgen receptor; AR-V7; prostate cancer; ddPCR; CTC
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Ma, Y.; Luk, A.; Young, F.P.; Lynch, D.; Chua, W.; Balakrishnar, B.; de Souza, P.; Becker, T.M. Droplet Digital PCR Based Androgen Receptor Variant 7 (AR-V7) Detection from Prostate Cancer Patient Blood Biopsies. Int. J. Mol. Sci. 2016, 17, 1264.

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