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Int. J. Mol. Sci. 2016, 17(8), 1211; doi:10.3390/ijms17081211

Glutathione Transferases Superfamily: Cold-Inducible Expression of Distinct GST Genes in Brassica oleracea

1
Department of Horticulture, Sunchon National University, 255, Jungang-ro, Suncheon 57922, Korea
2
Plant Systems Engineering Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahangno, Daejeon 34141, Korea
3
Department of Agricultural Education, Sunchon National University, 255, Jungang-ro, Suncheon 57922, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Jianhua Zhu
Received: 8 June 2016 / Revised: 15 July 2016 / Accepted: 15 July 2016 / Published: 27 July 2016
(This article belongs to the Section Molecular Botany)
View Full-Text   |   Download PDF [7029 KB, uploaded 27 July 2016]   |  

Abstract

Plants, as sessile organisms, can suffer serious growth and developmental consequences under cold stress conditions. Glutathione transferases (GSTs, EC 2.5.1.18) are ubiquitous and multifunctional conjugating proteins, which play a major role in stress responses by preventing oxidative damage by reactive oxygen species (ROS). Currently, understanding of their function(s) during different biochemical and signaling pathways under cold stress condition remain unclear. In this study, using combined computational strategy, we identified 65 Brassica oleracea glutathione transferases (BoGST) and characterized them based on evolutionary analysis into 11 classes. Inter-species and intra-species duplication was evident between BoGSTs and Arabidopsis GSTs. Based on localization analyses, we propose possible pathways in which GST genes are involved during cold stress. Further, expression analysis of the predicted putative functions for GST genes were investigated in two cold contrasting genotypes (cold tolerance and susceptible) under cold condition, most of these genes were highly expressed at 6 h and 1 h in the cold tolerant (CT) and cold susceptible (CS) lines, respectively. Overall, BoGSTU19, BoGSTU24, BoGSTF10 are candidate genes highly expressed in B. oleracea. Further investigation of GST superfamily in B. oleracea will aid in understanding complex mechanism underlying cold tolerance in plants. View Full-Text
Keywords: cold; glutathione transferase (GST); Brassica oleracea; contrasting genotypes; tolerance cold; glutathione transferase (GST); Brassica oleracea; contrasting genotypes; tolerance
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Vijayakumar, H.; Thamilarasan, S.K.; Shanmugam, A.; Natarajan, S.; Jung, H.-J.; Park, J.-I.; Kim, H.; Chung, M.-Y.; Nou, I.-S. Glutathione Transferases Superfamily: Cold-Inducible Expression of Distinct GST Genes in Brassica oleracea. Int. J. Mol. Sci. 2016, 17, 1211.

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