Next Article in Journal
Non-Ligand-Induced Dimerization is Sufficient to Initiate the Signalling and Endocytosis of EGF Receptor
Next Article in Special Issue
Molecular Signals in Nodulation Control
Previous Article in Journal
Influences of Electromagnetic Energy on Bio-Energy Transport through Protein Molecules in Living Systems and Its Experimental Evidence
Previous Article in Special Issue
Molecular Signals Controlling the Inhibition of Nodulation by Nitrate in Medicago truncatula
Article Menu
Issue 8 (August) cover image

Export Article

Open AccessCommunication
Int. J. Mol. Sci. 2016, 17(8), 1197; doi:10.3390/ijms17081197

Expression Analysis of PIN Genes in Root Tips and Nodules of Medicago truncatula

Department of Botany, Faculty of Agriculture and Biology, Warsaw University of Life Sciences—SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Academic Editors: Peter M. Gresshoff and Brett Ferguson
Received: 6 May 2016 / Revised: 7 June 2016 / Accepted: 15 July 2016 / Published: 25 July 2016
(This article belongs to the Special Issue Molecular Signals in Nodulation Control)
View Full-Text   |   Download PDF [2269 KB, uploaded 25 July 2016]   |  

Abstract

Polar auxin transport is dependent on the family of PIN-formed proteins (PINs), which are membrane transporters of anionic indole-3-acetic acid (IAA). It is assumed that polar auxin transport may be essential in the development and meristematic activity maintenance of Medicago truncatula (M. truncatula) root nodules. However, little is known about the involvement of specific PIN proteins in M. truncatula nodulation. Using real-time quantitative PCR, we analyzed the expression patterns of all previously identified MtPIN genes and compared them between root nodules and root tips of M. truncatula. Our results demonstrated significant differences in the expression level of all 11 genes (MtPIN1MtPIN11) between examined organs. Interestingly, MtPIN9 was the only PIN gene with higher expression level in root nodules compared to root tips. This result is the first indication of PIN9 transporter potential involvement in M. truncatula nodulation. Moreover, relatively high expression level in root nodules was attributed to MtPINs encoding orthologs of Arabidopsis thaliana PIN5 subclade. PIN proteins from this subclade have been found to localize in the endoplasmic reticulum, which may indicate that the development and meristematic activity maintenance of M. truncatula root nodules is associated with intracellular homeostasis of auxins level and their metabolism in the endoplasmic reticulum. View Full-Text
Keywords: Medicago truncatula; root nodule; auxin; PIN Medicago truncatula; root nodule; auxin; PIN
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Sańko-Sawczenko, I.; Łotocka, B.; Czarnocka, W. Expression Analysis of PIN Genes in Root Tips and Nodules of Medicago truncatula. Int. J. Mol. Sci. 2016, 17, 1197.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top