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Int. J. Mol. Sci. 2016, 17(7), 1139; doi:10.3390/ijms17071139

Global Transcriptomic Analysis Reveals the Mechanism of Phelipanche aegyptiaca Seed Germination

Key Laboratory at Universities of Xinjiang Uygur Autonomous Region for Oasis Agricultural Pest Management and Plant Protection Resource Utilization, Shihezi University, Shihezi 832003, China
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Academic Editor: Setsuko Komatsu
Received: 10 May 2016 / Revised: 10 July 2016 / Accepted: 11 July 2016 / Published: 15 July 2016
(This article belongs to the Section Molecular Botany)
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Abstract

Phelipanche aegyptiaca is one of the most destructive root parasitic plants of Orobanchaceae. This plant has significant impacts on crop yields worldwide. Conditioned and host root stimulants, in particular, strigolactones, are needed for unique seed germination. However, no extensive study on this phenomenon has been conducted because of insufficient genomic information. Deep RNA sequencing, including de novo assembly and functional annotation was performed on P. aegyptiaca germinating seeds. The assembled transcriptome was used to analyze transcriptional dynamics during seed germination. Key gene categories involved were identified. A total of 274,964 transcripts were determined, and 53,921 unigenes were annotated according to the NR, GO, COG, KOG, and KEGG databases. Overall, 5324 differentially expressed genes among dormant, conditioned, and GR24-treated seeds were identified. GO and KEGG enrichment analyses demonstrated numerous DEGs related to DNA, RNA, and protein repair and biosynthesis, as well as carbohydrate and energy metabolism. Moreover, ABA and ethylene were found to play important roles in this process. GR24 application resulted in dramatic changes in ABA and ethylene-associated genes. Fluridone, a carotenoid biosynthesis inhibitor, alone could induce P. aegyptiaca seed germination. In addition, conditioning was probably not the indispensable stage for P. aegyptiaca, because the transcript level variation of MAX2 and KAI2 genes (relate to strigolactone signaling) was not up-regulated by conditioning treatment. View Full-Text
Keywords: Phelipanche aegyptiaca; seed germination; ABA; strigolactones; GR24; transcriptome sequencing Phelipanche aegyptiaca; seed germination; ABA; strigolactones; GR24; transcriptome sequencing
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MDPI and ACS Style

Yao, Z.; Tian, F.; Cao, X.; Xu, Y.; Chen, M.; Xiang, B.; Zhao, S. Global Transcriptomic Analysis Reveals the Mechanism of Phelipanche aegyptiaca Seed Germination. Int. J. Mol. Sci. 2016, 17, 1139.

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