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Int. J. Mol. Sci. 2016, 17(5), 795; doi:10.3390/ijms17050795

Cold-Induced Browning Dynamically Alters the Expression Profiles of Inflammatory Adipokines with Tissue Specificity in Mice

1,2,†
,
1,2,3,†
,
1,2
,
1,2
and
1,2,*
1
Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Xi’an Jiaotong University Health Science Center, Xi’an 710061, China
2
Key Laboratory of Environment and Genes Related to Diseases, Xi’an Jiaotong University, Ministry of Education of China, Xi’an 710061, China
3
Department of Prosthodontics, Stomatological Hospital, College of Stomatology, Xi’an Jiaotong University, Xi’an 710061, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: M. Denis Girard
Received: 15 April 2016 / Revised: 12 May 2016 / Accepted: 18 May 2016 / Published: 23 May 2016
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
View Full-Text   |   Download PDF [3831 KB, uploaded 23 May 2016]   |  

Abstract

Cold exposure or β3-adrenoceptor agonist treatment induces the adipose tissues remodeling, relevant for beige adipogenesis within white adipose tissue (WAT). It remains unclear whether this process influences inflammatory adipokines expression in adipose tissues. We determine the temporal profile of cold or β3-adrenoceptor agonist (CL316,243)-induced changes in the expression of inflammatory adipokines in adipose tissues in mice or primary mice adipocytes. Male C57BL/6J mice at eight weeks old were exposed to 4 °C for 1–5 days. Interscapular brown adipose tissue (iBAT), inguinal subcutaneous WAT (sWAT) and epididymal WAT (eWAT) were harvested for gene and protein expression analysis. In addition, cultured primary mice brown adipocyte (BA) and white adipocyte (WA) treated with or without CL316,243 were harvested for gene expression analysis. The inflammatory adipokines expressed significantly higher in WAT than BAT at baseline. They were rapidly changed in iBAT, while down-regulated in sWAT and up-regulated in eWAT during the cold acclimation. Upon CL316,243 treatment, detected inflammatory adipokines except Leptin were transiently increased in both BA and WA. Our in vivo and in vitro data demonstrate that the browning process alters the inflammatory adipokines expression in adipose tissues, which is acutely responded to in iBAT, dynamically decreased in sWAT whilst increased in eWAT for compensation. View Full-Text
Keywords: inflammatory adipokines; beige cells; cold exposure; β3-adrenoceptor agonist; depot-specificity inflammatory adipokines; beige cells; cold exposure; β3-adrenoceptor agonist; depot-specificity
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MDPI and ACS Style

Luo, X.; Jia, R.; Zhang, Q.; Sun, B.; Yan, J. Cold-Induced Browning Dynamically Alters the Expression Profiles of Inflammatory Adipokines with Tissue Specificity in Mice. Int. J. Mol. Sci. 2016, 17, 795.

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