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Int. J. Mol. Sci. 2016, 17(5), 632; doi:10.3390/ijms17050632

Advantages and Pitfalls of Mass Spectrometry Based Metabolome Profiling in Systems Biology

Max Planck Institute for Molecular Genetics, Ihnestraße 63-73, 14195 Berlin, Germany
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Author to whom correspondence should be addressed.
Academic Editor: Ting-Li (Morgan) Han
Received: 22 March 2016 / Revised: 19 April 2016 / Accepted: 21 April 2016 / Published: 27 April 2016
(This article belongs to the Special Issue Metabolomic Technologies in Medicine)
View Full-Text   |   Download PDF [230 KB, uploaded 29 April 2016]

Abstract

Mass spectrometry-based metabolome profiling became the method of choice in systems biology approaches and aims to enhance biological understanding of complex biological systems. Genomics, transcriptomics, and proteomics are well established technologies and are commonly used by many scientists. In comparison, metabolomics is an emerging field and has not reached such high-throughput, routine and coverage than other omics technologies. Nevertheless, substantial improvements were achieved during the last years. Integrated data derived from multi-omics approaches will provide a deeper understanding of entire biological systems. Metabolome profiling is mainly hampered by its diversity, variation of metabolite concentration by several orders of magnitude and biological data interpretation. Thus, multiple approaches are required to cover most of the metabolites. No software tool is capable of comprehensively translating all the data into a biologically meaningful context yet. In this review, we discuss the advantages of metabolome profiling and main obstacles limiting progress in systems biology. View Full-Text
Keywords: metabolome profiling; mass spectrometry; nuclear magnetic resonance (NMR) spectroscopy; systems biology; multiple reaction monitoring (MRM); LC-MS; derivatization metabolome profiling; mass spectrometry; nuclear magnetic resonance (NMR) spectroscopy; systems biology; multiple reaction monitoring (MRM); LC-MS; derivatization
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Aretz, I.; Meierhofer, D. Advantages and Pitfalls of Mass Spectrometry Based Metabolome Profiling in Systems Biology. Int. J. Mol. Sci. 2016, 17, 632.

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