Environmental Conditions Influence Induction of Key ABC-Transporter Genes Affecting Glyphosate Resistance Mechanism in Conyza canadensis
Abstract
:1. Introduction
2. Results
2.1. Shikimate Measurements
2.2. Expression Analysis of ABC-Transporter-Like Genes and EPSPS Gene
2.2.1. Relative Expression of M10, M11 and EPSPS Genes at 24 °C
2.2.2. Relative Expression of M10, M11 and EPSPS Genes at 35 °C
2.2.3. Relative Expression of M10, M11 and EPSPS Genes at 8 °C
3. Discussion
4. Experimental Section
4.1. Plant Material
4.2. Herbicide and Temperature Treatments
4.3. Shikimate Measurement
4.4. RNA Isolation, cDNA Synthesis and qRT-PCRexperiments
4.5. Statistical Analysis
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Tani, E.; Chachalis, D.; Travlos, I.S.; Bilalis, D. Environmental Conditions Influence Induction of Key ABC-Transporter Genes Affecting Glyphosate Resistance Mechanism in Conyza canadensis. Int. J. Mol. Sci. 2016, 17, 342. https://doi.org/10.3390/ijms17040342
Tani E, Chachalis D, Travlos IS, Bilalis D. Environmental Conditions Influence Induction of Key ABC-Transporter Genes Affecting Glyphosate Resistance Mechanism in Conyza canadensis. International Journal of Molecular Sciences. 2016; 17(4):342. https://doi.org/10.3390/ijms17040342
Chicago/Turabian StyleTani, Eleni, Demosthenis Chachalis, Ilias S. Travlos, and Dimitrios Bilalis. 2016. "Environmental Conditions Influence Induction of Key ABC-Transporter Genes Affecting Glyphosate Resistance Mechanism in Conyza canadensis" International Journal of Molecular Sciences 17, no. 4: 342. https://doi.org/10.3390/ijms17040342