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Int. J. Mol. Sci. 2016, 17(3), 352; doi:10.3390/ijms17030352

Spinal Cord T-Cell Infiltration in the Rat Spared Nerve Injury Model: A Time Course Study

Pain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV) and University of Lausanne, 1011 Lausanne, Switzerland
Department of Fundamental Neurosciences, University of Lausanne, 1005 Lausanne, Switzerland
This author passed away.
Authors to whom correspondence should be addressed.
Academic Editor: Irmgard Tegeder
Received: 7 December 2015 / Revised: 26 February 2016 / Accepted: 29 February 2016 / Published: 9 March 2016
(This article belongs to the Special Issue Molecular and Cellular Mechanisms of Pain)
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The immune system is involved in the development of neuropathic pain. In particular, the infiltration of T-lymphocytes into the spinal cord following peripheral nerve injury has been described as a contributor to sensory hypersensitivity. We used the spared nerve injury (SNI) model of neuropathic pain in Sprague Dawley adult male rats to assess proliferation, and/or protein/gene expression levels for microglia (Iba1), T-lymphocytes (CD2) and cytotoxic T-lymphocytes (CD8). In the dorsal horn ipsilateral to SNI, Iba1 and BrdU stainings revealed microglial reactivity and proliferation, respectively, with different durations. Iba1 expression peaked at D4 and D7 at the mRNA and protein level, respectively, and was long-lasting. Proliferation occurred almost exclusively in Iba1 positive cells and peaked at D2. Gene expression observation by RT-qPCR array suggested that T-lymphocytes attracting chemokines were upregulated after SNI in rat spinal cord but only a few CD2/CD8 positive cells were found. A pronounced infiltration of CD2/CD8 positive T-cells was seen in the spinal cord injury (SCI) model used as a positive control for lymphocyte infiltration. Under these experimental conditions, we show early and long-lasting microglia reactivity in the spinal cord after SNI, but no lymphocyte infiltration was found. View Full-Text
Keywords: peripheral nerve injury; SNI (spared nerve injury); SCI (spinal cord injury); microglia; lymphocytes; neuropathic pain peripheral nerve injury; SNI (spared nerve injury); SCI (spinal cord injury); microglia; lymphocytes; neuropathic pain

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MDPI and ACS Style

Gattlen, C.; Clarke, C.B.; Piller, N.; Kirschmann, G.; Pertin, M.; Decosterd, I.; Gosselin, R.-D.; Suter, M.R. Spinal Cord T-Cell Infiltration in the Rat Spared Nerve Injury Model: A Time Course Study. Int. J. Mol. Sci. 2016, 17, 352.

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