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Int. J. Mol. Sci. 2016, 17(3), 329; doi:10.3390/ijms17030329

Evaluation of the Cytotoxic Behavior of Fungal Extracellular Synthesized Ag Nanoparticles Using Confocal Laser Scanning Microscope

1
Nanotechnology and Advanced Materials Central Lab, Agriculture Research Center, PO Box 588 Orman, Giza 12619, Egypt
2
Faculty of Women for Art, Science & Education, Ain Shams University, PO Box 11757 Alkurba, Cairo 11341, Egypt
3
Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
4
Materials Science and Technology Program, College of Arts and Sciences, Qatar University, PO Box 2713, Doha, Qatar
5
Department of Chemistry, University of Texas at Austin, Austin, TX 78712, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Michael Routledge
Received: 20 October 2015 / Revised: 19 February 2016 / Accepted: 19 February 2016 / Published: 3 March 2016
(This article belongs to the Collection Bioactive Nanoparticles)
View Full-Text   |   Download PDF [1844 KB, uploaded 3 March 2016]   |  

Abstract

Silver nanoparticles have been synthesized by subjecting a reaction medium to a Fusarium oxysporum biomass at 28 °C for 96 h. The biosynthesized Ag nanoparticles were characterized on the basis of their anticipated peak at 405 nm using UV-Vis-NIR spectroscopy. Structural confirmation was evident from the characteristic X-ray diffraction (XRD) pattern, high-resolution transmission electron Microscopy (HRTEM) and the particle size analyzer. The Ag nanoparticles were of dimension 40 ± 5 nm and spherical in shape. The study mainly focused on using the confocal laser scanning microscope (CLSM) to examine the cytotoxic activities of fungal synthesized Ag nanoparticles on a human breast carcinoma cell line MCF7 cell, which featured remarkable vacuolation, thus indicating a potent cytotoxic activity. View Full-Text
Keywords: silver nanoparticles; cytotoxicity; breast cancer; confocal laser scanning microcopy silver nanoparticles; cytotoxicity; breast cancer; confocal laser scanning microcopy
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MDPI and ACS Style

Salaheldin, T.A.; Husseiny, S.M.; Al-Enizi, A.M.; Elzatahry, A.; Cowley, A.H. Evaluation of the Cytotoxic Behavior of Fungal Extracellular Synthesized Ag Nanoparticles Using Confocal Laser Scanning Microscope. Int. J. Mol. Sci. 2016, 17, 329.

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