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Int. J. Mol. Sci. 2016, 17(3), 329; doi:10.3390/ijms17030329

Evaluation of the Cytotoxic Behavior of Fungal Extracellular Synthesized Ag Nanoparticles Using Confocal Laser Scanning Microscope

Nanotechnology and Advanced Materials Central Lab, Agriculture Research Center, PO Box 588 Orman, Giza 12619, Egypt
Faculty of Women for Art, Science & Education, Ain Shams University, PO Box 11757 Alkurba, Cairo 11341, Egypt
Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
Materials Science and Technology Program, College of Arts and Sciences, Qatar University, PO Box 2713, Doha, Qatar
Department of Chemistry, University of Texas at Austin, Austin, TX 78712, USA
Author to whom correspondence should be addressed.
Academic Editor: Michael Routledge
Received: 20 October 2015 / Revised: 19 February 2016 / Accepted: 19 February 2016 / Published: 3 March 2016
(This article belongs to the Collection Bioactive Nanoparticles)
View Full-Text   |   Download PDF [1844 KB, uploaded 3 March 2016]   |  


Silver nanoparticles have been synthesized by subjecting a reaction medium to a Fusarium oxysporum biomass at 28 °C for 96 h. The biosynthesized Ag nanoparticles were characterized on the basis of their anticipated peak at 405 nm using UV-Vis-NIR spectroscopy. Structural confirmation was evident from the characteristic X-ray diffraction (XRD) pattern, high-resolution transmission electron Microscopy (HRTEM) and the particle size analyzer. The Ag nanoparticles were of dimension 40 ± 5 nm and spherical in shape. The study mainly focused on using the confocal laser scanning microscope (CLSM) to examine the cytotoxic activities of fungal synthesized Ag nanoparticles on a human breast carcinoma cell line MCF7 cell, which featured remarkable vacuolation, thus indicating a potent cytotoxic activity. View Full-Text
Keywords: silver nanoparticles; cytotoxicity; breast cancer; confocal laser scanning microcopy silver nanoparticles; cytotoxicity; breast cancer; confocal laser scanning microcopy

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Salaheldin, T.A.; Husseiny, S.M.; Al-Enizi, A.M.; Elzatahry, A.; Cowley, A.H. Evaluation of the Cytotoxic Behavior of Fungal Extracellular Synthesized Ag Nanoparticles Using Confocal Laser Scanning Microscope. Int. J. Mol. Sci. 2016, 17, 329.

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