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Int. J. Mol. Sci. 2016, 17(12), 1902; doi:10.3390/ijms17121902

Rapid, Sensitive Detection of Bartonella quintana by Loop-Mediated Isothermal Amplification of the groEL Gene

1,2,†
,
3,4,†
,
1,†
,
2,†
,
2,5
and
2,*
1
Clinical Laboratory of Peking University Shougang Hospital, Beijing100144, China
2
State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, China
3
School of Tropical and Laboratory Medicine, Hainan Medical University, Haikou 571199, China
4
School of Life Science, Shanxi University, Taiyuan 030006, China
5
Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Stephen A. Bustin
Received: 2 September 2016 / Revised: 8 November 2016 / Accepted: 10 November 2016 / Published: 1 December 2016
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
View Full-Text   |   Download PDF [7469 KB, uploaded 1 December 2016]   |  

Abstract

Trench fever, caused by Bartonella quintana, is recognized as a re-emerging and neglected disease. Rapid and sensitive detection approaches are urgently required to monitor and help control B. quintana infections. Here, loop-mediated isothermal amplification (LAMP), which amplifies target DNA at a fixed temperature with high sensitivity, specificity and rapidity, was employed to detect B. quintana. Thirty-six strains, including 10 B. quintana, 13 other Bartonella spp., and 13 other common pathogens, were applied to verify and evaluate the LAMP assay. The specificity of the LAMP assay was 100%, and the limit of detection was 125 fg/reaction. The LAMP assay was compared with qPCR in the examination of 100 rhesus and 20 rhesus-feeder blood samples; the diagnostic accuracy was found to be 100% when LAMP was compared to qPCR, but the LAMP assay was significantly more sensitive (p < 0.05). Thus, LAMP methodology is a useful for diagnosis of trench fever in humans and primates, especially in low-resource settings, because of its rapid, sensitive detection that does not require sophisticated equipment. View Full-Text
Keywords: Bartonella; trench fever; re-emerging pathogen; detection Bartonella; trench fever; re-emerging pathogen; detection
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Hu, S.; Niu, L.; Luo, L.; Song, X.; Sun, J.; Liu, Q. Rapid, Sensitive Detection of Bartonella quintana by Loop-Mediated Isothermal Amplification of the groEL Gene. Int. J. Mol. Sci. 2016, 17, 1902.

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