Cloning and Expression of Ecdysone Receptor and Retinoid X Receptor from Procambarus clarkii: Induction by Eyestalk Ablation
Abstract
:1. Introduction
2. Results
2.1. Cloning of Full Length cDNAs of PcEcR and PcRXR
2.2. Sequence Alignments and Phylogenic Trees of PcEcR and PcRXR
2.3. Expression of PcEcR and PcRXR in Different Tissues
2.4. The Induction Expression of PcEcR and PcRXR after Eyestalk Ablation
3. Discussion
3.1. Analysis of PcEcR and PcRXR
3.2. Expression in Different Tissues and Synergistic Expression of PcEcR and PcRXR in Different Tissues
3.3. Induction of PcEcR and PcRXR after ESA in Different Tissues
4. Material and Methods
4.1. Animal Collection, Preparation of Total RNA, and cDNA Synthesis
4.2. Cloning and Sequencing of Full-Length PcEcR and PcRXR cDNA
4.3. Sequence Alignments and Phylogenetic Analysis
4.4. Quantitation of PcEcR and PcRXR Transcripts by Real-Time PCR
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Primer | Sequence (5′ to 3′) | Primer Description |
---|---|---|
PcEcR-3a-Outer | CTCACAGAATTGCGAACCCTT | 3′ RACE Primer for first round |
PcEcR-3a-Inner | CACCCAGGACCCACTTTCAG | 3′ RACE Primer for second round |
PcEcR-5a-Outer | GAGATGTTACTGCTTCCCAC | 5′ RACE Primer for first round |
PcEcR-5a-Inner | ATCCTTTGGGTTTACAATCA | 5′ RACE Primer for second round |
PcRXR-3a-Outer | CAATACTGGCATCGGTTCCT | 3′ RACE Primer for first round |
PcRXR-3a-Inner | CCGCCATTGGTGGTGGAGAA | 3′ RACE Primer for second round |
PcRXR-5a-Outer | AAAACAAGGAAGTAGTTGGC | 5′ RACE Primer for first round |
PcRXR-5a-Inner | TAAAACTCAAGGAAACTGATG | 5′ RACE Primer for second round |
Rt-PcEcR-F | CCTGTGAGGGATGCAAAGGT | FWD primer for EsEcR expression |
Rt-PcEcR-R | GCATTGAGACTCGGGAACCA | RVS primer for EsEcR expression |
Rt-PcRXR-F | CCTTCACCATTGGGTCGAGT | FWD primer for EsEcR expression |
Rt-PcRXR-R | AGCTGTAGACGCCATAGTGC | RVS primer for EsEcR expression |
Pc18S-F | ATCACGTCTCTGACCGCAAG | FWD primer for 18S expression |
Pc18S-R | GACACTTGAAAGATGCGGCG | RVS primer for 18S expression |
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Dai, T.-H.; Sserwadda, A.; Song, K.; Zang, Y.-N.; Shen, H.-S. Cloning and Expression of Ecdysone Receptor and Retinoid X Receptor from Procambarus clarkii: Induction by Eyestalk Ablation. Int. J. Mol. Sci. 2016, 17, 1739. https://doi.org/10.3390/ijms17101739
Dai T-H, Sserwadda A, Song K, Zang Y-N, Shen H-S. Cloning and Expression of Ecdysone Receptor and Retinoid X Receptor from Procambarus clarkii: Induction by Eyestalk Ablation. International Journal of Molecular Sciences. 2016; 17(10):1739. https://doi.org/10.3390/ijms17101739
Chicago/Turabian StyleDai, Tian-Hao, Ali Sserwadda, Kun Song, Ya-Nan Zang, and Huai-Shun Shen. 2016. "Cloning and Expression of Ecdysone Receptor and Retinoid X Receptor from Procambarus clarkii: Induction by Eyestalk Ablation" International Journal of Molecular Sciences 17, no. 10: 1739. https://doi.org/10.3390/ijms17101739